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Journal of Bacteriology, May 2001, p. 3256-3260, Vol. 183, No. 10
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.10.3256-3260.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Effects of Mutations in the Pseudomonas putida miaA Gene: Regulation of the trpE and trpGDC Operons in P. putida by Attenuation

Igor Olekhnovich1,2,dagger and Gary N. Gussin2,*

Department of Microbiology, Belarus State University, Minsk 220050, Belarus,1 and Department of Biological Sciences, University of Iowa, Iowa City, Iowa 522422

Received 30 October 2000/Accepted 23 February 2001

Tn5 insertion mutants defective in regulation of the Pseudomonas putida trpE and trpGDC operons by tryptophan were found to contain insertions in the P. putida miaA gene, whose product (in Escherichia coli) modifies tRNATrp and is required for attenuation. Nucleotide sequences upstream of trpE and trpG encode putative leader peptides similar in sequence to leader peptides found in other bacterial species, and the phenotypes of the mutants strongly suggest that transcription of these operons is regulated solely by attenuation.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242. Phone: (319) 335-1113. Fax: (319) 335-1069. E-mail: gary-gussin{at}uiowa.edu.

dagger Present address: Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908.

Journal of Bacteriology, May 2001, p. 3256-3260, Vol. 183, No. 10
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.10.3256-3260.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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