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Journal of Bacteriology, June 2001, p. 3276-3281, Vol. 183, No. 11
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.11.3276-3281.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of a Three-Component Vanillate O-Demethylase from Moorella thermoacetica

Devendra Naidu and Stephen W. Ragsdale*

Department of Biochemistry, Beadle Center, University of Nebraska---Lincoln, Lincoln, Nebraska 68588-0664

Received 23 October 2000/Accepted 13 March 2001

The Moorella thermoacetica aromatic O-demethylase was characterized as an inducible three-component system with similarity to the methanogenic methanol, methylamine, and methanethiol methyltransferases and to the O-demethylase system from Acetobacterium dehalogenans. MtvB catalyzes methyl transfer from a phenylmethylether to the cobalt center of MtvC, a corrinoid protein. MtvA catalyzes transmethylation from MtvC to tetrahydrofolate, forming methyltetrahydrofolate. Cobalamin can substitute for MtvC.

* Corresponding author. Mailing address: Department of Biochemistry, Beadle Center, University of Nebraska---Lincoln, Lincoln, NE 68588-0664. Phone: (402) 472-2943. Fax: (402) 472-7842. E-mail: sragsdale1{at}unl.edu.

Journal of Bacteriology, June 2001, p. 3276-3281, Vol. 183, No. 11
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.11.3276-3281.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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