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Journal of Bacteriology, June 2001, p. 3521-3525, Vol. 183, No. 11
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.11.3521-3525.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

RegG, a CcpA Homolog, Participates in Regulation of Amylase-Binding Protein A Gene (abpA) Expression in Streptococcus gordonii

Jeffrey D. Rogers and Frank A. Scannapieco^*

Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, New York 14214

Received 20 November 2000/Accepted 12 January 2001

The amylase-binding protein A (AbpA) of Streptococcus gordonii was found to be undetectable in supernatants of mid-log-phase cultures containing >1% glucose but abundant in supernatants of cultures made with brain heart infusion (BHI), which contains 0.2% glucose. A 10-fold decrease in the level of abpA mRNA in S. gordonii cells cultured in BHI was noted after the addition of glucose to 1%. Analysis of the abpA sequence revealed a potential catabolite responsive element CRE 153 bp downstream of the putative translational start site. A catabolite control protein A gene (ccpA) homolog from S. gordonii, designated regG, was cloned. A regG mutant strain demonstrated moderately less repression of abpA transcription in the presence of 1% glucose. Diauxic growth with glucose and lactose was not affected in the RegG mutant compared to the wild-type parental strain. These results suggest that while RegG plays a role in abpA expression, other mechanisms of catabolite repression are present.

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^* Corresponding author. Mailing address: 109 Foster Hall, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY 14214. Phone: (716) 829-3373. Fax: (716) 829-3942. E-mail: fas1{at}acsu.buffalo.edu.

Present address: Department of Periodontics, School of Dentistry, Virginia Commonwealth University, Richmond, VA 23298-0566.

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Journal of Bacteriology, June 2001, p. 3521-3525, Vol. 183, No. 11
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.11.3521-3525.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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