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Journal of Bacteriology, June 2001, p. 3631-3635, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3631-3635.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Very-Short-Patch Repair in Escherichia coli Requires the dam Adenine Methylase

Derek C. Bell and Claire G. Cupples*

Biology Department, Concordia University, Montréal, Québec H3G 1M8, Canada

Received 11 December 2000/Accepted 2 April 2001

Strains of Escherichia coli which lack the dam-encoded adenine methylase are mutators due to a reduction in the efficiency of postreplication mismatch repair. In this study, we show that Dam- strains are also defective in very-short-patch repair, the system which corrects T/G mismatches arising from the deamination of 5-methylcytosine. This defect is associated with decreased levels of Vsr, the endonuclease which initiates short-patch repair. We also show that production of the dcm-encoded cytosine methylase is unaffected in Dam- strains. Since the dcm and vsr genes are cotranscribed, the regulation of Vsr by Dam is probably posttranscriptional.

* Corresponding author. Mailing address: Biology Department, Concordia University, 1455 de Maisonneuve Blvd., W., Montréal, Québec H3G 1M8, Canada. Phone: (514) 848-3390, Fax: (514) 848-2881. E-mail: claire{at}vax2.concordia.ca.

Journal of Bacteriology, June 2001, p. 3631-3635, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3631-3635.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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