This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gartemann, K.-H.
Right arrow Articles by Eichenlaub, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gartemann, K.-H.
Right arrow Articles by Eichenlaub, R.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2001, p. 3729-3736, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3729-3736.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Isolation and Characterization of IS1409, an Insertion Element of 4-Chlorobenzoate-Degrading Arthrobacter sp. Strain TM1, and Development of a System for Transposon Mutagenesis

Karl-Heinz Gartemann and Rudolf Eichenlaub*

Fakultät für Biologie, Lehrstuhl für Mikrobiologie/Gentechnologie, Universität Bielefeld, Bielefeld, Germany

Received 18 August 2000/Accepted 28 March 2001

A new insertion element of 1,449 bp with 25-bp perfect terminal repeats, designated IS1409, was identified in the chromosome of 4-chlorobenzoate-degrading Arthrobacter sp. strain TM1 NCIB12013. Upon insertion, IS1409 causes a target duplication of 8 bp. IS1409 carries only a single open reading frame of 435 codons encoding the transposase TnpA. Both TnpA and the overall organization of IS1409 are highly similar to those of some related insertion elements of the ISL3 group (J. Mahillon and M. Chandler, Microbiol. Mol. Biol. Rev. 62:725-774, 1998). IS1409 was also found in other 4-chlorobenzoate-degrading Arthrobacter strains and Micrococcus luteus. Based on IS1409, a series of transposons carrying resistance genes for chloramphenicol and gentamicin were constructed. These transposons were used to demonstrate transposition events in vivo and to mutagenize Arthrobacter sp. strains.

* Corresponding author. Mailing address: Department of Microbiology/Gene Technology, University of Bielefeld, Universitaetsstr., 33615 Bielefeld, Germany. Phone: 49(0)5211065558. Fax: 49(0)5211066015. E-mail: eichenlaub{at}biologie.uni-bielefeld.de.

Journal of Bacteriology, June 2001, p. 3729-3736, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3729-3736.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ganas, P., Brandsch, R. (2009). Uptake of L-nicotine and of 6-hydroxy-L-nicotine by Arthrobacter nicotinovorans and by Escherichia coli is mediated by facilitated diffusion and not by passive diffusion or active transport. Microbiology 155: 1866-1877 [Abstract] [Full Text]  
  • Gartemann, K.-H., Abt, B., Bekel, T., Burger, A., Engemann, J., Flugel, M., Gaigalat, L., Goesmann, A., Grafen, I., Kalinowski, J., Kaup, O., Kirchner, O., Krause, L., Linke, B., McHardy, A., Meyer, F., Pohle, S., Ruckert, C., Schneiker, S., Zellermann, E.-M., Puhler, A., Eichenlaub, R., Kaiser, O., Bartels, D. (2008). The Genome Sequence of the Tomato-Pathogenic Actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 Reveals a Large Island Involved in Pathogenicity. J. Bacteriol. 190: 2138-2149 [Abstract] [Full Text]  
  • Chae, J.-C., Zylstra, G. J. (2006). 4-Chlorobenzoate Uptake in Comamonas sp. Strain DJ-12 Is Mediated by a Tripartite ATP-Independent Periplasmic Transporter. J. Bacteriol. 188: 8407-8412 [Abstract] [Full Text]  
  • Sachelaru, P., Schiltz, E., Brandsch, R. (2006). A Functional mobA Gene for Molybdopterin Cytosine Dinucleotide Cofactor Biosynthesis Is Required for Activity and Holoenzyme Assembly of the Heterotrimeric Nicotine Dehydrogenases of Arthrobacter nicotinovorans.. Appl. Environ. Microbiol. 72: 5126-5131 [Abstract] [Full Text]  
  • Sandu, C., Chiribau, C.-B., Sachelaru, P., Brandsch, R. (2005). Plasmids for Nicotine-Dependent and -Independent Gene Expression in Arthrobacter nicotinovorans and Other Arthrobacter Species. Appl. Environ. Microbiol. 71: 8920-8924 [Abstract] [Full Text]  
  • Nordin, K., Unell, M., Jansson, J. K. (2005). Novel 4-Chlorophenol Degradation Gene Cluster and Degradation Route via Hydroxyquinol in Arthrobacter chlorophenolicus A6. Appl. Environ. Microbiol. 71: 6538-6544 [Abstract] [Full Text]  
  • Overhage, J., Sielker, S., Homburg, S., Parschat, K., Fetzner, S. (2005). Identification of large linear plasmids in Arthrobacter spp. encoding the degradation of quinaldine to anthranilate. Microbiology 151: 491-500 [Abstract] [Full Text]  
  • Igloi, G. L., Brandsch, R. (2003). Sequence of the 165-Kilobase Catabolic Plasmid pAO1 from Arthrobacter nicotinovorans and Identification of a pAO1-Dependent Nicotine Uptake System. J. Bacteriol. 185: 1976-1986 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»