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Journal of Bacteriology, June 2001, p. 3737-3741, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3737-3741.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Amplification of Mutator Cells in a Population as a Result of Horizontal Transfer

Pauline Funchain, Annie Yeung, Jean Stewart, Wendy M. Clendenin, and Jeffrey H. Miller*

Department of Microbiology and Molecular Genetics, University of California, Los Angeles, California 90095

Received 6 December 2000/Accepted 2 April 2001

Mutator cells that lack the mismatch repair system (MMR-) occur at rates of 10-5 or less in laboratory populations started from wild-type cells. We show that after selection for recombinants in an interspecies mating between Salmonella enterica serovar Typhimurium and Escherichia coli, the percentage of MMR- cells rises to several percent of the recombinant population, and after a second successive mating and selection, greater than 95% of the recombinants are MMR-. Coupling a single cross and selection with either mutagenesis or selection for spontaneous mutants also results in a dramatic increase in MMR- cells. We discuss how horizontal transfer can result in mutator strains during adaptive evolution.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, University of California, 405 Hilgard Ave., Los Angeles, CA 90095. Phone: (310) 825-8460. Fax: (310) 206-3088. E-mail: jhmiller{at}mbi.ucla.edu.

Journal of Bacteriology, June 2001, p. 3737-3741, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3737-3741.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.