This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, J. T.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2001, p. 3842-3847, Vol. 183, No. 13
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.13.3842-3847.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of a Dedicated Recycling Pathway for Anhydro-N-Acetylmuramic Acid and N-Acetylglucosamine Derived from Escherichia coli Cell Wall Murein

James T. Park*

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111

Received 7 March 2001/Accepted 6 April 2001

Turnover and recycling of the cell wall murein represent a major metabolic pathway of Escherichia coli. It is known that E. coli efficiently reuses, i.e., recycles, its murein tripeptide, L-alanyl-gamma -D-glutamyl-meso-diaminopimelate, to form new murein. However, the question of whether the cells also recycle the amino sugar moieties of cell wall murein has remained unanswered. It is demonstrated here that E. coli recycles the N-acetylglucosamine present in cell wall murein degradation products for de novo murein and lipopolysaccharide synthesis. Furthermore, E. coli also recycles the anhydro-N-acetylmuramic acid moiety by first converting it into N-acetylglucosamine. Based on the results obtained by studying mutants unable to recycle amino sugars, the pathway for recycling is revealed.

* Mailing address: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6753. Fax: (617) 636-0337. E-mail: james.park{at}tufts.edu.

Journal of Bacteriology, July 2001, p. 3842-3847, Vol. 183, No. 13
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.13.3842-3847.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Plumbridge, J. (2009). An Alternative Route for Recycling of N-Acetylglucosamine from Peptidoglycan Involves the N-Acetylglucosamine Phosphotransferase System in Escherichia coli. J. Bacteriol. 191: 5641-5647 [Abstract] [Full Text]  
  • Iida, A., Ohnishi, Y., Horinouchi, S. (2009). Identification and characterization of target genes of the GinI/GinR quorum-sensing system in Gluconacetobacter intermedius. Microbiology 155: 3021-3032 [Abstract] [Full Text]  
  • Jaeger, T., Mayer, C. (2008). The Transcriptional Factors MurR and Catabolite Activator Protein Regulate N-Acetylmuramic Acid Catabolism in Escherichia coli. J. Bacteriol. 190: 6598-6608 [Abstract] [Full Text]  
  • Park, J. T., Uehara, T. (2008). How Bacteria Consume Their Own Exoskeletons (Turnover and Recycling of Cell Wall Peptidoglycan). Microbiol. Mol. Biol. Rev. 72: 211-227 [Abstract] [Full Text]  
  • Lan, G., Wolgemuth, C. W., Sun, S. X. (2007). Z-ring force and cell shape during division in rod-like bacteria. Proc. Natl. Acad. Sci. USA 104: 16110-16115 [Abstract] [Full Text]  
  • Wexler, H. M. (2007). Bacteroides: the Good, the Bad, and the Nitty-Gritty. Clin. Microbiol. Rev. 20: 593-621 [Abstract] [Full Text]  
  • Uehara, T., Park, J. T. (2007). An Anhydro-N-Acetylmuramyl-L-Alanine Amidase with Broad Specificity Tethered to the Outer Membrane of Escherichia coli. J. Bacteriol. 189: 5634-5641 [Abstract] [Full Text]  
  • Lim, J.-H., Kim, M.-S., Kim, H.-E., Yano, T., Oshima, Y., Aggarwal, K., Goldman, W. E., Silverman, N., Kurata, S., Oh, B.-H. (2006). Structural Basis for Preferential Recognition of Diaminopimelic Acid-type Peptidoglycan by a Subset of Peptidoglycan Recognition Proteins. J. Biol. Chem. 281: 8286-8295 [Abstract] [Full Text]  
  • Uehara, T., Suefuji, K., Jaeger, T., Mayer, C., Park, J. T. (2006). MurQ Etherase Is Required by Escherichia coli in Order To Metabolize Anhydro-N-Acetylmuramic Acid Obtained either from the Environment or from Its Own Cell Wall. J. Bacteriol. 188: 1660-1662 [Abstract] [Full Text]  
  • Jaeger, T., Arsic, M., Mayer, C. (2005). Scission of the Lactyl Ether Bond of N-Acetylmuramic Acid by Escherichia coli "Etherase". J. Biol. Chem. 280: 30100-30106 [Abstract] [Full Text]  
  • Uehara, T., Suefuji, K., Valbuena, N., Meehan, B., Donegan, M., Park, J. T. (2005). Recycling of the Anhydro-N-Acetylmuramic Acid Derived from Cell Wall Murein Involves a Two-Step Conversion to N-Acetylglucosamine-Phosphate. J. Bacteriol. 187: 3643-3649 [Abstract] [Full Text]  
  • Alvarez-Anorve, L. I., Calcagno, M. L., Plumbridge, J. (2005). Why Does Escherichia coli Grow More Slowly on Glucosamine than on N-Acetylglucosamine? Effects of Enzyme Levels and Allosteric Activation of GlcN6P Deaminase (NagB) on Growth Rates. J. Bacteriol. 187: 2974-2982 [Abstract] [Full Text]  
  • Mahapatra, S., Scherman, H., Brennan, P. J., Crick, D. C. (2005). N Glycolylation of the Nucleotide Precursors of Peptidoglycan Biosynthesis of Mycobacterium spp. Is Altered by Drug Treatment. J. Bacteriol. 187: 2341-2347 [Abstract] [Full Text]  
  • Mengin-Lecreulx, D., Lemaitre, B. (2005). Structure and metabolism of peptidoglycan and molecular requirements allowing its detection by the Drosophila innate immune system. Innate Immunity 11: 105-111 [Abstract]  
  • Brigham, C. J., Malamy, M. H. (2005). Characterization of the RokA and HexA Broad-Substrate-Specificity Hexokinases from Bacteroides fragilis and Their Role in Hexose and N-Acetylglucosamine Utilization. J. Bacteriol. 187: 890-901 [Abstract] [Full Text]  
  • Sohanpal, B. K., El-Labany, S., Lahooti, M., Plumbridge, J. A., Blomfield, I. C. (2004). Integrated regulatory responses of fimB to N-acetylneuraminic (sialic) acid and GlcNAc in Escherichia coli K-12. Proc. Natl. Acad. Sci. USA 101: 16322-16327 [Abstract] [Full Text]  
  • Uehara, T., Park, J. T. (2004). The N-Acetyl-D-Glucosamine Kinase of Escherichia coli and Its Role in Murein Recycling. J. Bacteriol. 186: 7273-7279 [Abstract] [Full Text]  
  • de Pedro, M. A., Grunfelder, C. G., Schwarz, H. (2004). Restricted Mobility of Cell Surface Proteins in the Polar Regions of Escherichia coli. J. Bacteriol. 186: 2594-2602 [Abstract] [Full Text]  
  • Dahl, U., Jaeger, T., Nguyen, B. T., Sattler, J. M., Mayer, C. (2004). Identification of a Phosphotransferase System of Escherichia coli Required for Growth on N-Acetylmuramic Acid. J. Bacteriol. 186: 2385-2392 [Abstract] [Full Text]  
  • Vincent, F., Yates, D., Garman, E., Davies, G. J., Brannigan, J. A. (2004). The Three-dimensional Structure of the N-Acetylglucosamine-6-phosphate Deacetylase, NagA, from Bacillus subtilis: A MEMBER OF THE UREASE SUPERFAMILY. J. Biol. Chem. 279: 2809-2816 [Abstract] [Full Text]  
  • Uehara, T., Park, J. T. (2003). Identification of MpaA, an Amidase in Escherichia coli That Hydrolyzes the {gamma}-D-Glutamyl-meso-Diaminopimelate Bond in Murein Peptides. J. Bacteriol. 185: 679-682 [Abstract] [Full Text]  
  • Uehara, T., Park, J. T. (2002). Role of the Murein Precursor UDP-N-Acetylmuramyl-L-Ala-{gamma}-D-Glu- meso-Diaminopimelic Acid-D-Ala-D-Ala in Repression of {beta}-Lactamase Induction in Cell Division Mutants. J. Bacteriol. 184: 4233-4239 [Abstract] [Full Text]  
  • Faure, D. (2002). The Family-3 Glycoside Hydrolases: from Housekeeping Functions to Host-Microbe Interactions. Appl. Environ. Microbiol. 68: 1485-1490 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»