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Journal of Bacteriology, August 2001, p. 4395-4404, Vol. 183, No. 15
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.15.4395-4404.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transposition of IS1397 in the Family Enterobacteriaceae and First Characterization of ISKpn1, a New Insertion Sequence Associated with Klebsiella pneumoniae Palindromic Units

Caroline Wilde, Sophie Bachellier, Maurice Hofnung, and Jean-Marie Clément^*

Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA 1444, Institut Pasteur, 75724 Paris Cedex 15, France

Received 23 February 2001/Accepted 15 May 2001

IS1397 and ISKpn1 are IS3 family members which are specifically inserted into the loop of palindromic units (PUs). IS1397 is shown to transpose into PUs with sequences close or identical to the Escherichia coli consensus, even in other enterobacteria (Salmonella enterica serovar Typhimurium, Klebsiella pneumoniae, and Klebsiella oxytoca). Moreover, we show that homologous intergenic regions containing PUs constitute IS1397 transpositional hot spots, despite bacterial interspersed mosaic element structures that differ among the three species. ISKpn1, described here for the first time, is specific for PUs from K. pneumoniae, in which we discovered it. A sequence comparison between the two insertion sequences allowed us to define a motif possibly accounting for their specificity.

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^* Corresponding author. Mailing address: Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA 1444, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: (33) 01 40 61 32 88. Fax: (33) 01 45 68 88 34. E-mail: jclement{at}pasteur.fr.

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Journal of Bacteriology, August 2001, p. 4395-4404, Vol. 183, No. 15
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.15.4395-4404.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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