Competence Repression under Oxygen Limitation through the Two-Component MicAB Signal-Transducing System in Streptococcus pneumoniae and Involvement of the PAS Domain of MicB

doi:10.1128/JB.183.15.4599-4608.2001

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Journal of Bacteriology, August 2001, p. 4599-4608, Vol. 183, No. 15
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.15.4599-4608.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Competence Repression under Oxygen Limitation through the Two-Component MicAB Signal-Transducing System in Streptococcus pneumoniae and Involvement of the PAS Domain of MicB

José R. Echenique and Marie-Claude Trombe^*

Laboratoire de Genétique et Physiologie Bactérienne, E.A. 3036, Centre Hospitalo Universitaire de Rangueil, Université Paul Sabatier, 31403 Toulouse Cedex, France

Received 21 February 2001/Accepted 14 May 2001

In Streptococcus pneumoniae, a fermentative aerotolerant and catalase-deficient human pathogen, oxidases with molecular oxygenas substrate are important for virulence and for competence. Thesignal-transducing two-component systems CiaRH and ComDE mediatethe response to oxygen, culminating in competence. In this workwe show that the two-component MicAB system, whose MicB kinasecarries a PAS domain, is also involved in competence repressionunder oxygen limitation. Autophosphorylation of recombinant MicBand phosphotransfer to recombinant MicA have been demonstrated.Mutational analysis and in vitro assays showed that the C-terminalpart of the protein and residue L100 in the N-terminal cap ofits PAS domain are both crucial for autokinase activity in vitro.Although no insertion mutation in micA was obtained, expressionof the mutated allele micA59DA did not change bacterial growthand overcame competence repression under microaerobiosis. Thiswas related to a strong instability of MicA59DA-PO₄ in vitro.Thus, mutations which either reduced the stability of MicA-PO₄or abolished kinase activity in MicB were related to competencederepression under microaerobiosis, suggesting that MicA-PO₄ isinvolved in competence repression when oxygen becomes limiting.The micAB genes are flanked by mutY and orfC. MutY is an adenineglycosylase involved in the repair of oxidized pyrimidines. OrfCshows the features of a metal binding protein. We did not obtaininsertion mutation in orfC, suggesting its requirement for growth.It is proposed that MicAB, with its PAS motif, may belong to aset of functions important in the protection of the cell againstoxidative stress, including the control ofcompetence.

^* Corresponding author. Mailing address: Laboratoire de Genétique et Physiologie Bacterienne, E.A. 3036, Centre Hospitalo Universitairede Rangueil, Université Paul Sabatier, 31403 Toulouse Cedex,France. Phone: (33)61-322974. Fax: (33)61-322620. E-mail: trombe{at}cict.fr.

Present address: Departmento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, PabellónArgentina, Ciudad Universitaria, CP 5000 Córdoba,Argentina.

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