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Journal of Bacteriology, August 2001, p. 4932-4937, Vol. 183, No. 16
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.16.4932-4937.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Iron-Dependent Transcription of the frpB Gene of Helicobacter pylori Is Controlled by the Fur Repressor Protein

Isabel Delany,¹ Ana Beatriz F. Pacheco,^1, Gunther Spohn,¹ Rino Rappuoli,¹ and Vincenzo Scarlato^1,2,*

Department of Molecular Biology, IRIS Chiron S.p.A., 53100 Siena,¹ and Department of Biology, University of Bologna, 40126 Bologna,² Italy

Received 9 April 2001/Accepted 1 June 2001

We have overexpressed and purified the Helicobacter pylori Fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake (frpB and ceuE) and oxygen radical detoxification (katA and tsaA). DNase I footprint analysis showed that Fur binds specifically to a high-affinity site overlapping the P_frpB promoter and to low-affinity sites located upstream from promoters within both the frpB-katA and ceuE-tsaA intergenic regions. Construction of an isogenic fur mutant indicated that Fur regulates transcription from the P_frpB promoter in response to iron. In contrast, no effect by either Fur or iron was observed for the other promoters.

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^* Corresponding author. Mailing address: Department of Molecular Biology, IRIS, Chiron S.p.A., Via Fiorentina 1, 53100 Siena, Italy. Phone: 39 0577 243565. Fax: 39 0577 243564. E-mail: enzo_scarlato{at}chiron.it.

Present address: Biophysics Institute, University of Rio de Janeiro, Rio de Janeiro, Brazil.

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Journal of Bacteriology, August 2001, p. 4932-4937, Vol. 183, No. 16
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.16.4932-4937.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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