This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Delany, I.
Right arrow Articles by Scarlato, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Delany, I.
Right arrow Articles by Scarlato, V.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2001, p. 4932-4937, Vol. 183, No. 16
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.16.4932-4937.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Iron-Dependent Transcription of the frpB Gene of Helicobacter pylori Is Controlled by the Fur Repressor Protein

Isabel Delany,1 Ana Beatriz F. Pacheco,1,dagger Gunther Spohn,1 Rino Rappuoli,1 and Vincenzo Scarlato1,2,*

Department of Molecular Biology, IRIS Chiron S.p.A., 53100 Siena,1 and Department of Biology, University of Bologna, 40126 Bologna,2 Italy

Received 9 April 2001/Accepted 1 June 2001

We have overexpressed and purified the Helicobacter pylori Fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake (frpB and ceuE) and oxygen radical detoxification (katA and tsaA). DNase I footprint analysis showed that Fur binds specifically to a high-affinity site overlapping the PfrpB promoter and to low-affinity sites located upstream from promoters within both the frpB-katA and ceuE-tsaA intergenic regions. Construction of an isogenic fur mutant indicated that Fur regulates transcription from the PfrpB promoter in response to iron. In contrast, no effect by either Fur or iron was observed for the other promoters.

* Corresponding author. Mailing address: Department of Molecular Biology, IRIS, Chiron S.p.A., Via Fiorentina 1, 53100 Siena, Italy. Phone: 39 0577 243565. Fax: 39 0577 243564. E-mail: enzo_scarlato{at}chiron.it.

dagger Present address: Biophysics Institute, University of Rio de Janeiro, Rio de Janeiro, Brazil.

Journal of Bacteriology, August 2001, p. 4932-4937, Vol. 183, No. 16
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.16.4932-4937.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Carpenter, B. M., Whitmire, J. M., Merrell, D. S. (2009). This Is Not Your Mother's Repressor: the Complex Role of Fur in Pathogenesis. Infect. Immun. 77: 2590-2601 [Full Text]  
  • Danielli, A., Romagnoli, S., Roncarati, D., Costantino, L., Delany, I., Scarlato, V. (2009). Growth Phase and Metal-Dependent Transcriptional Regulation of the fecA Genes in Helicobacter pylori. J. Bacteriol. 191: 3717-3725 [Abstract] [Full Text]  
  • Ledala, N., Pearson, S. L., Wilkinson, B. J., Jayaswal, R. K. (2007). Molecular characterization of the Fur protein of Listeria monocytogenes. Microbiology 153: 1103-1111 [Abstract] [Full Text]  
  • Lee, H.-J., Bang, S. H., Lee, K.-H., Park, S.-J. (2007). Positive Regulation of fur Gene Expression via Direct Interaction of Fur in a Pathogenic Bacterium, Vibrio vulnificus. J. Bacteriol. 189: 2629-2636 [Abstract] [Full Text]  
  • Danielli, A., Roncarati, D., Delany, I., Chiarini, V., Rappuoli, R., Scarlato, V. (2006). In Vivo Dissection of the Helicobacter pylori Fur Regulatory Circuit by Genome-Wide Location Analysis. J. Bacteriol. 188: 4654-4662 [Abstract] [Full Text]  
  • Alamuri, P., Mehta, N., Burk, A., Maier, R. J. (2006). Regulation of the Helicobacter pylori Fe-S Cluster Synthesis Protein NifS by Iron, Oxidative Stress Conditions, and Fur.. J. Bacteriol. 188: 5325-5330 [Abstract] [Full Text]  
  • Gancz, H., Censini, S., Merrell, D. S. (2006). Iron and pH Homeostasis Intersect at the Level of Fur Regulation in the Gastric Pathogen Helicobacter pylori. Infect. Immun. 74: 602-614 [Abstract] [Full Text]  
  • Ernst, F. D., Homuth, G., Stoof, J., Mader, U., Waidner, B., Kuipers, E. J., Kist, M., Kusters, J. G., Bereswill, S., van Vliet, A. H. M. (2005). Iron-Responsive Regulation of the Helicobacter pylori Iron-Cofactored Superoxide Dismutase SodB Is Mediated by Fur. J. Bacteriol. 187: 3687-3692 [Abstract] [Full Text]  
  • Ernst, F. D., Bereswill, S., Waidner, B., Stoof, J., Mader, U., Kusters, J. G., Kuipers, E. J., Kist, M., van Vliet, A. H. M., Homuth, G. (2005). Transcriptional profiling of Helicobacter pylori Fur- and iron-regulated gene expression. Microbiology 151: 533-546 [Abstract] [Full Text]  
  • Merrell, D. S., Thompson, L. J., Kim, C. C., Mitchell, H., Tompkins, L. S., Lee, A., Falkow, S. (2003). Growth Phase-Dependent Response of Helicobacter pylori to Iron Starvation. Infect. Immun. 71: 6510-6525 [Abstract] [Full Text]  
  • Cooksley, C., Jenks, P. J., Green, A., Cockayne, A., Logan, R. P. H., Hardie, K. R. (2003). NapA protects Helicobacter pylori from oxidative stress damage, and its production is influenced by the ferric uptake regulator. J Med Microbiol 52: 461-469 [Abstract] [Full Text]  
  • van Vliet, A. H. M., Stoof, J., Poppelaars, S. W., Bereswill, S., Homuth, G., Kist, M., Kuipers, E. J., Kusters, J. G. (2003). Differential Regulation of Amidase- and Formamidase-mediated Ammonia Production by the Helicobacter pylori Fur Repressor. J. Biol. Chem. 278: 9052-9057 [Abstract] [Full Text]  
  • Harris, A. G., Hinds, F. E., Beckhouse, A. G., Kolesnikow, T., Hazell, S. L. (2002). Resistance to hydrogen peroxide in Helicobacter pylori: role of catalase (KatA) and Fur, and functional analysis of a novel gene product designated 'KatA-associated protein', KapA (HP0874). Microbiology 148: 3813-3825 [Abstract] [Full Text]  
  • Waidner, B., Greiner, S., Odenbreit, S., Kavermann, H., Velayudhan, J., Stahler, F., Guhl, J., Bisse, E., van Vliet, A. H. M., Andrews, S. C., Kusters, J. G., Kelly, D. J., Haas, R., Kist, M., Bereswill, S. (2002). Essential Role of Ferritin Pfr in Helicobacter pylori Iron Metabolism and Gastric Colonization. Infect. Immun. 70: 3923-3929 [Abstract] [Full Text]  
  • Bijlsma, J. J. E., Waidner, B., Vliet, A. H. M. v., Hughes, N. J., Hag, S., Bereswill, S., Kelly, D. J., Vandenbroucke-Grauls, C. M. J. E., Kist, M., Kusters, J. G. (2002). The Helicobacter pylori Homologue of the Ferric Uptake Regulator Is Involved in Acid Resistance. Infect. Immun. 70: 606-611 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»