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Journal of Bacteriology, September 2001, p. 4979-4984, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.4979-4984.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cellobiose Uptake in the Hyperthermophilic Archaeon Pyrococcus furiosus Is Mediated by an Inducible, High-Affinity ABC Transporter

Sonja M. Koning, Marieke G. L. Elferink,dagger Wil N. Konings, and Arnold J. M. Driessen*

Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 90750 AA Haren, The Netherlands

Received 26 January 2001/Accepted 12 June 2001

The hyperthermophilic archaeon Pyrococcus furiosus can utilize different beta -glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity (Km = 175 nM) and involves an inducible binding protein-dependent transport system. The cellobiose binding protein (CbtA) was purified from P. furiosus membranes to homogeneity as a 70-kDa glycoprotein. CbtA not only binds cellobiose but also cellotriose, cellotetraose, cellopentaose, laminaribiose, laminaritriose, and sophorose. The cbtA gene was cloned and functionally expressed in Escherichia coli. cbtA belongs to a gene cluster that encodes a transporter that belongs to the Opp family of ABC transporters.

* Corresponding author. Mailing address: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands. Phone: 31 50 3632164. Fax: 31 50 3632154. E-mail: a.j.m.driessen{at}biol.rug.nl.

dagger Present address: Department of Pharmacokinetics and Drug Delivery, Groningen Institute for Drug Exploration (Guide), University Center for Pharmacy, 9713 AV Groningen, The Netherlands.

Journal of Bacteriology, September 2001, p. 4979-4984, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.4979-4984.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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