This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koning, S. M.
Right arrow Articles by Driessen, A. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koning, S. M.
Right arrow Articles by Driessen, A. J. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2001, p. 4979-4984, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.4979-4984.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cellobiose Uptake in the Hyperthermophilic Archaeon Pyrococcus furiosus Is Mediated by an Inducible, High-Affinity ABC Transporter

Sonja M. Koning, Marieke G. L. Elferink,dagger Wil N. Konings, and Arnold J. M. Driessen*

Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 90750 AA Haren, The Netherlands

Received 26 January 2001/Accepted 12 June 2001

The hyperthermophilic archaeon Pyrococcus furiosus can utilize different beta -glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity (Km = 175 nM) and involves an inducible binding protein-dependent transport system. The cellobiose binding protein (CbtA) was purified from P. furiosus membranes to homogeneity as a 70-kDa glycoprotein. CbtA not only binds cellobiose but also cellotriose, cellotetraose, cellopentaose, laminaribiose, laminaritriose, and sophorose. The cbtA gene was cloned and functionally expressed in Escherichia coli. cbtA belongs to a gene cluster that encodes a transporter that belongs to the Opp family of ABC transporters.

* Corresponding author. Mailing address: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands. Phone: 31 50 3632164. Fax: 31 50 3632154. E-mail: a.j.m.driessen{at}biol.rug.nl.

dagger Present address: Department of Pharmacokinetics and Drug Delivery, Groningen Institute for Drug Exploration (Guide), University Center for Pharmacy, 9713 AV Groningen, The Netherlands.

Journal of Bacteriology, September 2001, p. 4979-4984, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.4979-4984.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mardanov, A. V., Ravin, N. V., Svetlitchnyi, V. A., Beletsky, A. V., Miroshnichenko, M. L., Bonch-Osmolovskaya, E. A., Skryabin, K. G. (2009). Metabolic Versatility and Indigenous Origin of the Archaeon Thermococcus sibiricus, Isolated from a Siberian Oil Reservoir, as Revealed by Genome Analysis. Appl. Environ. Microbiol. 75: 4580-4588 [Abstract] [Full Text]  
  • Anderson, I., Rodriguez, J., Susanti, D., Porat, I., Reich, C., Ulrich, L. E., Elkins, J. G., Mavromatis, K., Lykidis, A., Kim, E., Thompson, L. S., Nolan, M., Land, M., Copeland, A., Lapidus, A., Lucas, S., Detter, C., Zhulin, I. B., Olsen, G. J., Whitman, W., Mukhopadhyay, B., Bristow, J., Kyrpides, N. (2008). Genome Sequence of Thermofilum pendens Reveals an Exceptional Loss of Biosynthetic Pathways without Genome Reduction. J. Bacteriol. 190: 2957-2965 [Abstract] [Full Text]  
  • Ferrand, Y., Crump, M. P., Davis, A. P. (2007). A Synthetic Lectin Analog for Biomimetic Disaccharide Recognition. Science 318: 619-622 [Abstract] [Full Text]  
  • Szabo, Z., Sani, M., Groeneveld, M., Zolghadr, B., Schelert, J., Albers, S.-V., Blum, P., Boekema, E. J., Driessen, A. J. M. (2007). Flagellar Motility and Structure in the Hyperthermoacidophilic Archaeon Sulfolobus solfataricus. J. Bacteriol. 189: 4305-4309 [Abstract] [Full Text]  
  • Bevers, L. E., Hagedoorn, P.-L., Krijger, G. C., Hagen, W. R. (2006). Tungsten Transport Protein A (WtpA) in Pyrococcus furiosus: the First Member of a New Class of Tungstate and Molybdate Transporters.. J. Bacteriol. 188: 6498-6505 [Abstract] [Full Text]  
  • Lee, H.-S., Shockley, K. R., Schut, G. J., Conners, S. B., Montero, C. I., Johnson, M. R., Chou, C.-J., Bridger, S. L., Wigner, N., Brehm, S. D., Jenney, F. E. Jr., Comfort, D. A., Kelly, R. M., Adams, M. W. W. (2006). Transcriptional and Biochemical Analysis of Starch Metabolism in the Hyperthermophilic Archaeon Pyrococcus furiosus. J. Bacteriol. 188: 2115-2125 [Abstract] [Full Text]  
  • Nanavati, D. M., Thirangoon, K., Noll, K. M. (2006). Several Archaeal Homologs of Putative Oligopeptide-Binding Proteins Encoded by Thermotoga maritima Bind Sugars. Appl. Environ. Microbiol. 72: 1336-1345 [Abstract] [Full Text]  
  • Conners, S. B., Montero, C. I., Comfort, D. A., Shockley, K. R., Johnson, M. R., Chhabra, S. R., Kelly, R. M. (2005). An Expression-Driven Approach to the Prediction of Carbohydrate Transport and Utilization Regulons in the Hyperthermophilic Bacterium Thermotoga maritima. J. Bacteriol. 187: 7267-7282 [Abstract] [Full Text]  
  • Eichler, J., Adams, M. W. W. (2005). Posttranslational Protein Modification in Archaea. Microbiol. Mol. Biol. Rev. 69: 393-425 [Abstract] [Full Text]  
  • Silva, Z., Sampaio, M.-M., Henne, A., Bohm, A., Gutzat, R., Boos, W., da Costa, M. S., Santos, H. (2005). The High-Affinity Maltose/Trehalose ABC Transporter in the Extremely Thermophilic Bacterium Thermus thermophilus HB27 Also Recognizes Sucrose and Palatinose. J. Bacteriol. 187: 1210-1218 [Abstract] [Full Text]  
  • Nguyen, T. N., Ejaz, A. D., Brancieri, M. A., Mikula, A. M., Nelson, K. E., Gill, S. R., Noll, K. M. (2004). Whole-Genome Expression Profiling of Thermotoga maritima in Response to Growth on Sugars in a Chemostat. J. Bacteriol. 186: 4824-4828 [Abstract] [Full Text]  
  • Nanavati, D., Noll, K. M., Romano, A. H. (2002). Periplasmic maltose- and glucose-binding protein activities in cell-free extracts of Thermotoga maritima. Microbiology 148: 3531-3537 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»