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Journal of Bacteriology, September 2001, p. 4979-4984, Vol. 183, No. 17
Department of Microbiology, Groningen
Biomolecular Sciences and Biotechnology Institute, University of
Groningen, 90750 AA Haren, The Netherlands
Received 26 January 2001/Accepted 12 June 2001
The hyperthermophilic archaeon Pyrococcus furiosus
can utilize different
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.17.4979-4984.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Cellobiose Uptake in the Hyperthermophilic Archaeon
Pyrococcus furiosus Is Mediated by an Inducible,
High-Affinity ABC Transporter

-glucosides, like cellobiose and laminarin.
Cellobiose uptake occurs with high affinity
(Km = 175 nM) and involves an
inducible binding protein-dependent transport system. The cellobiose binding protein (CbtA) was purified from P. furiosus
membranes to homogeneity as a 70-kDa glycoprotein. CbtA not only binds
cellobiose but also cellotriose, cellotetraose, cellopentaose,
laminaribiose, laminaritriose, and sophorose. The cbtA
gene was cloned and functionally expressed in Escherichia coli.
cbtA belongs to a gene cluster that encodes a transporter that
belongs to the Opp family of ABC transporters.
*
Corresponding author. Mailing address: Department of
Microbiology, Groningen Biomolecular Sciences and Biotechnology
Institute, University of Groningen, P.O. Box 14, 9750 AA Haren, The
Netherlands. Phone: 31 50 3632164. Fax: 31 50 3632154. E-mail:
a.j.m.driessen{at}biol.rug.nl.
Present address: Department of Pharmacokinetics and Drug Delivery,
Groningen Institute for Drug Exploration (Guide), University Center for
Pharmacy, 9713 AV Groningen, The Netherlands.
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