This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamamoto, H.
Right arrow Articles by Sekiguchi, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamamoto, H.
Right arrow Articles by Sekiguchi, J.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2001, p. 5110-5121, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.5110-5121.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Regulation of the glv Operon in Bacillus subtilis: YfiA (GlvR) Is a Positive Regulator of the Operon That Is Repressed through CcpA and cre

Hiroki Yamamoto,1 Masakuni Serizawa,1 John Thompson,2 and Junichi Sekiguchi1,*

Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386-8567, Japan,1 and Microbial Biochemistry and Genetics Unit, Oral Infection and Immunity Branch, NIDCR, National Institutes of Health, Bethesda, Maryland 208922

Received 8 March 2001/Accepted 5 June 2001

Maltose metabolism and the regulation of the glv operon of Bacillus subtilis, comprising three genes, glvA (6-phospho-alpha -glucosidase), yfiA (now designated glvR), and glvC (EIICB transport protein), were investigated. Maltose dissimilation was dependent primarily upon the glv operon, and insertional inactivation of either glvA, glvR, or glvC markedly inhibited growth on the disaccharide. A second system (MalL) contributed to a minor extent to maltose metabolism. Northern blotting revealed two transcripts corresponding to a monocistronic mRNA of glvA and a polycistronic mRNA of glvA-glvR-glvC. Primer extension analysis showed that both transcripts started at the same base (G) located 26 bp upstream of the 5' end of glvA. When glvR was placed under control of the spac promoter, expression of the glv operon was dependent upon the presence of isopropyl-beta -D-thiogalactopyranoside (IPTG). In regulatory studies, the promoter sequence of the glv operon was fused to lacZ and inserted into the amyE locus, and the resultant strain (AMGLV) was then transformed with a citrate-controlled glvR plasmid, pHYCM2VR. When cultured in Difco sporulation medium containing citrate, this transformant [AMGLV(pHYCM2VR)] expressed LacZ activity, but synthesis of LacZ was repressed by glucose. In an isogenic strain, [AMGLVCR(pHYCM2VR)], except for a mutation in the sequence of a catabolite-responsive element (cre), LacZ activity was expressed in the presence of citrate and glucose. Insertion of a citrate-controlled glvR plasmid at the amyE locus of ccpA+ and ccpA mutant organisms yielded strains AMCMVR and AMCMVRCC, respectively. In the presence of both glucose and citrate, AMCMVR failed to express the glv operon, whereas under the same conditions high-level expression of both mRNA transcripts was found in strain AMCMVRCC. Collectively, our findings suggest that GlvR (the product of the glvR gene) is a positive regulator of the glv operon and that glucose exerts its effect via catabolite repression requiring both CcpA and cre.


* Corresponding author. Mailing address: Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386-8567, Japan. Phone: 81 268 21 5344. Fax: 81 268 21 5345. E-mail: jsekigu{at}giptc.shinshu-u.ac.jp.


Journal of Bacteriology, September 2001, p. 5110-5121, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.5110-5121.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Daddaoua, A., Krell, T., Ramos, J.-L. (2009). Regulation of Glucose Metabolism in Pseudomonas: THE PHOSPHORYLATIVE BRANCH AND ENTNER-DOUDOROFF ENZYMES ARE REGULATED BY A REPRESSOR CONTAINING A SUGAR ISOMERASE DOMAIN. J. Biol. Chem. 284: 21360-21368 [Abstract] [Full Text]  
  • Jaeger, T., Mayer, C. (2008). The Transcriptional Factors MurR and Catabolite Activator Protein Regulate N-Acetylmuramic Acid Catabolism in Escherichia coli. J. Bacteriol. 190: 6598-6608 [Abstract] [Full Text]  
  • Kobayashi, K. (2007). Bacillus subtilis Pellicle Formation Proceeds through Genetically Defined Morphological Changes. J. Bacteriol. 189: 4920-4931 [Abstract] [Full Text]  
  • Schonert, S., Seitz, S., Krafft, H., Feuerbaum, E.-A., Andernach, I., Witz, G., Dahl, M. K. (2006). Maltose and Maltodextrin Utilization by Bacillus subtilis. J. Bacteriol. 188: 3911-3922 [Abstract] [Full Text]  
  • Gupta, A., Maranas, C. D., Albert, R. (2006). Elucidation of directionality for co-expressed genes: predicting intra-operon termination sites. Bioinformatics 22: 209-214 [Abstract] [Full Text]  
  • Thompson, J., Hess, S., Pikis, A. (2004). Genes malh and pagl of Clostridium acetobutylicum ATCC 824 Encode NAD+- and Mn2+-dependent Phospho-{alpha}-glucosidase(s). J. Biol. Chem. 279: 1553-1561 [Abstract] [Full Text]  
  • Barrangou, R., Altermann, E., Hutkins, R., Cano, R., Klaenhammer, T. R. (2003). Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus. Proc. Natl. Acad. Sci. USA 100: 8957-8962 [Abstract] [Full Text]  
  • Thompson, J., Robrish, S. A., Immel, S., Lichtenthaler, F. W., Hall, B. G., Pikis, A. (2001). Metabolism of Sucrose and Its Five Linkage-isomeric alpha -D-Glucosyl-D-fructoses by Klebsiella pneumoniae. PARTICIPATION AND PROPERTIES OF SUCROSE-6-PHOSPHATE HYDROLASE AND PHOSPHO-alpha -GLUCOSIDASE. J. Biol. Chem. 276: 37415-37425 [Abstract] [Full Text]