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Journal of Bacteriology, September 2001, p. 5203-5205, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.5203-5205.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Methanococcus jannaschii Generates L-Proline by Cyclization of L-Ornithine

Marion Graupner and Robert H. White*

Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308

Received 26 March 2001/Accepted 7 June 2001

Cell extracts of Methanococcus jannaschii have been shown to readily convert L-ornithine to L-proline. This cyclization reaction proceeds with the loss of only the C-2 nitrogen, as has been documented for ornithine cyclodeaminase (EC 4.3.1.12). Since no gene homologous to that coding for ornithine cyclodeaminase is present in the genome of M. jannaschii, these results indicate that proline biosynthesis in M. jannaschii is accomplished by a previously unrecognized enzyme.

* Corresponding author. Mailing address: Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0308. Phone: (540) 231-6605. Fax: (540) 231-9070. E-mail: rhwhite{at}vt.edu.

Journal of Bacteriology, September 2001, p. 5203-5205, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.5203-5205.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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