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 Previous Article

Journal of Bacteriology, September 2001, p. 5209-5212, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.5209-5212.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Dehalogenation of Dichloromethane by Dichloromethane Dehalogenase/Glutathione S-Transferase Leads to Formation of DNA Adducts

Martin F. Kayser and Stéphane Vuilleumier*

Institut für Mikrobiologie, ETH Zürich, CH-8092 Zürich, Switzerland

Received 28 March 2001/Accepted 12 June 2001

Formation of DNA adducts following conversion of dichloromethane by bacterial dichloromethane dehalogenase/glutathione S-transferase was demonstrated. Adducts included dichloromethane carbon and glutathione sulfur atoms. A reaction with DNA occurred preferentially at guanine bases. Increased DNA degradation in a polA mutant of Methylobacterium dichloromethanicum DM4 grown with dichloromethane confirmed the genotoxicity associated with dichloromethane degradation, suggesting an important role of DNA repair in the metabolism of halogenated, DNA-alkylating compounds by bacteria.

* Corresponding author. Mailing address: Institut für Mikrobiologie, ETH Zürich, Schmelzbergstr. 7, CH-8092 Zürich, Switzerland. Phone: 41-01-6323357. Fax: 41-01-6321148. E-mail: vuilleumier{at}micro.biol.ethz.ch.

Journal of Bacteriology, September 2001, p. 5209-5212, Vol. 183, No. 17
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.17.5209-5212.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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