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Journal of Bacteriology, September 2001, p. 5239-5247, Vol. 183, No. 18
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.18.5239-5247.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

DNA Microarray-Based Identification of Genes Controlled by Autoinducer 2-Stimulated Quorum Sensing in Escherichia coli

Matthew P. DeLisa,1,2 Chi-Fang Wu,3 Liang Wang,4 James J. Valdes,3 and William E. Bentley1,2,*

Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute,1 Department of Chemical Engineering2 and Department of Cell Biology and Molecular Genetics,4 University of Maryland, College Park, and U.S. Army Edgewood Research, Development, and Engineering Center, Aberdeen Proving Grounds, Aberdeen, Maryland3

Received 14 February 2001/Accepted 29 June 2001

Bacterial cell-to-cell communication facilitates coordinated expression of specific genes in a growth rate-II and cell density-dependent manner, a process known as quorum sensing. While the discovery of a diffusible Escherichia coli signaling pheromone, termed autoinducer 2 (AI-2), has been made along with several quorum sensing genes, the overall number and coordination of genes controlled by quorum sensing through the AI-2 signal has not been studied systematically. We investigated global changes in mRNA abundance elicited by the AI-2 signaling molecule through the use of a luxS mutant that was unable to synthesize AI-2. Remarkably, 242 genes, comprising ca. 5.6% of the E. coli genome, exhibited significant transcriptional changes (either induction or repression) in response to a 300-fold AI-2 signaling differential, with many of the identified genes displaying high induction levels (more than fivefold). Significant induction of ygeV, a putative sigma 54-dependent transcriptional activator, and yhbH, a sigma 54 modulating protein, suggests sigma 54 may be involved in E. coli quorum sensing.

* Corresponding author. Mailing address: Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, University of Maryland, College Park, MD 20742. Phone: (301) 405-4321. Fax: (301) 314-9075. E-mail: bentley{at}eng.umd.edu.

Journal of Bacteriology, September 2001, p. 5239-5247, Vol. 183, No. 18
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.18.5239-5247.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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