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Journal of Bacteriology, September 2001, p. 5257-5261, Vol. 183, No. 18
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.18.5257-5261.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Three Target Genes for the Transcriptional Activator Cat8p of Kluyveromyces lactis: Acetyl Coenzyme A Synthetase Genes KlACS1 and KlACS2 and Lactate Permease Gene KlJEN1

Tiziana Lodi,1 Michele Saliola,2 Claudia Donnini,1 and Paola Goffrini1,*

Istituto di Genetica, Parco Area delle Scienze 11-A, Università degli Studi di Parma, 43100 Parma,1 and Dipartimamento di Biologia Cellulare e dello Sviluppo, Università di Roma "La Sapienza," Piazzale A. Moro 5, 00185 Rome,2 Italy

Received 24 January 2001/Accepted 25 June 2001

The aerobic yeast Kluyveromyces lactis and the predominantly fermentative Saccharomyces cerevisiae share many of the genes encoding the enzymes of carbon and energy metabolism. The physiological features that distinguish the two yeasts appear to result essentially from different organization of regulatory circuits, in particular glucose repression and gluconeogenesis. We have isolated the KlCAT8 gene (a homologue of S. cerevisiae CAT8, encoding a DNA binding protein) as a multicopy suppressor of a fog1 mutation. The Fog1 protein is a homologue of the Snf1 complex components Gal83p, Sip1p, and Sip2p of S. cerevisiae. While CAT8 controls the key enzymes of gluconeogenesis in S. cerevisiae, KlCAT8 of K. lactis does not (I. Georis, J. J. Krijger, K. D. Breunig, and J. Vandenhaute, Mol. Gen. Genet. 264:193-203, 2000). We therefore examined possible targets of KlCat8p. We found that the acetyl coenzyme A synthetase genes, KlACS1 and KlACS2, were specifically regulated by KlCAT8, but very differently from the S. cerevisiae counterparts. KlACS1 was induced by acetate and lactate, while KlACS2 was induced by ethanol, both under the control of KlCAT8. Also, KlJEN1, encoding the lactate-inducible and glucose-repressible lactate permease, was found under a tight control of KlCAT8.

* Corresponding author. Mailing address: Istituto di Genetica, Università degli Studi di Parma, Parco Area delle Scienze 11-A, 43100 Parma, Italy. Phone: 39 0521 905602. Fax: 39 0521 905604. E-mail: goffrini{at}unipr.it.

Journal of Bacteriology, September 2001, p. 5257-5261, Vol. 183, No. 18
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.18.5257-5261.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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