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Journal of Bacteriology, September 2001, p. 5371-5375, Vol. 183, No. 18
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.18.5371-5375.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Hop Resistance in the Beer Spoilage Bacterium Lactobacillus brevis Is Mediated by the ATP-Binding Cassette Multidrug Transporter HorA

Kanta Sakamoto,1 Abelardo Margolles,2,dagger Hendrik W. van Veen,2,Dagger and Wil N. Konings2,*

Brewing Research & Development Laboratory, Asahi Breweries, Ltd., Moriya-machi, Kitasoma-gun, Ibaraki 302-0106, Japan,1 and Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands2

Received 21 November 2000/Accepted 12 June 2001

Lactobacillus brevis is a major contaminant of spoiled beer. The organism can grow in beer in spite of the presence of antibacterial hop compounds that give the beer a bitter taste. The hop resistance in L. brevis is, at least in part, dependent on the expression of the horA gene. The deduced amino acid sequence of HorA is 53% identical to that of LmrA, an ATP-binding cassette multidrug transporter in Lactococcus lactis. To study the role of HorA in hop resistance, HorA was functionally expressed in L. lactis as a hexa-histidine-tagged protein using the nisin-controlled gene expression system. HorA expression increased the resistance of L. lactis to hop compounds and cytotoxic drugs. Drug transport studies with L. lactis cells and membrane vesicles and with proteoliposomes containing purified HorA protein identified HorA as a new member of the ABC family of multidrug transporters.

* Corresponding author. Mailing address: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands. Phone: 31-50-363-21-50. Fax: 31-50-3632154. E-mail: w.n.konings{at}biol.rug.nel.

dagger Present address: Instituto de Productos Lacteos de Asturias, IPLA-CSIC, Ctra/Infiesto s/n, 33300 Villaviciosa, Asturias, Spain.

Dagger Present address: Department of Pharmacology, University of Cambridge, CB2 1QJ Cambridge, United Kingdom.

Journal of Bacteriology, September 2001, p. 5371-5375, Vol. 183, No. 18
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.18.5371-5375.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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