Alginate Overproduction Affects Pseudomonas aeruginosa Biofilm Structure and Function

doi:10.1128/JB.183.18.5395-5401.2001

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Journal of Bacteriology, September 2001, p. 5395-5401, Vol. 183, No. 18
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.18.5395-5401.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Alginate Overproduction Affects Pseudomonas aeruginosa Biofilm Structure and Function

Morten Hentzer,¹ Gail M. Teitzel,² Grant J. Balzer,² Arne Heydorn,¹ Søren Molin,¹ Michael Givskov,¹ and Matthew R. Parsek²^,^*

Department of Microbiology, Technical University of Denmark, 2800 Lyngby, Denmark,¹ and Department of Civil Engineering, Northwestern University, Evanston, Illinois 60208²

Received 20 February 2001/Accepted 15 June 2001

During the course of chronic cystic fibrosis (CF) infections, Pseudomonas aeruginosa undergoes a conversion to a mucoid phenotype,which is characterized by overproduction of the exopolysaccharidealginate. Chronic P. aeruginosa infections involve surface-attached,highly antibiotic-resistant communities of microorganisms organizedin biofilms. Although biofilm formation and the conversion tomucoidy are both important aspects of CF pathogenesis, the relationshipbetween them is at the present unclear. In this study, we reportthat the overproduction of alginate affects biofilm developmenton an abiotic surface. Biofilms formed by an alginate-overproducingstrain exhibit a highly structured architecture and are significantlymore resistant to the antibiotic tobramycin than a biofilm formedby an isogenic nonmucoid strain. These results suggest that animportant consequence of the conversion to mucoidy is an alteredbiofilm architecture that shows increasing resistance to antimicrobialtreatments.

^* Corresponding author. Mailing address: Environmental Health Engineering, Department of Civil Engineering, Northwestern University,2145 Sheridan Rd., Evanston, IL 60208. Phone: (847) 467-7445.Fax: (847) 491-4011. E-mail: m-parsek{at}nwu.edu.

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