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Journal of Bacteriology, October 2001, p. 5529-5534, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5529-5534.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Promoter Specificity Elements in Pseudomonas aeruginosa Quorum-Sensing-Controlled Genes

Marvin Whiteley and E. P. Greenberg*

Department of Microbiology, University of Iowa, Iowa City, Iowa 52242

Received 12 April 2001/Accepted 26 June 2001

The LasR-dependent and RhlR-dependent quorum-sensing systems are global regulators of gene expression in Pseudomonas aeruginosa. Previous studies have demonstrated that promoter elements of the quorum-sensing-controlled genes lasB and hcnABC are important in density-dependent regulation. We have identified LasR- and RhlR-dependent determinants in promoters of quorum-sensing-controlled genes qsc102, qsc117 (acpP), and qsc131 (phzA to -G) by in silico, deletion, point-mutational, and primer extension analyses. Each of these genes (in addition to lasI and rsaL) is activated by LasR, and qsc117 and qsc131 also respond to RhlR. Point mutations in the promoters of the LasR-specific gene, qsc102, relax specificity so that this promoter can respond to RhlR in addition to LasR. Our findings indicate that quorum-sensing-controlled promoters in P. aeruginosa are either specific for LasR or respond to both LasR and RhlR and that critical bases in the promoter elements determine specificity.

* Corresponding author. Mailing address: Department of Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7775. Fax: (319) 335-7949. E-mail: everett-greenberg{at}uiowa.edu.

Journal of Bacteriology, October 2001, p. 5529-5534, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5529-5534.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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