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Journal of Bacteriology, October 2001, p. 5580-5588, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5580-5588.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Expression of cspH, Encoding the Cold Shock Protein in Salmonella enterica Serovar Typhimurium UK-1

Bae Hoon Kim,1 Iel Soo Bang,1 Soo Youn Lee,1 Seong Karp Hong,1 Seong Ho Bang,2 In Soo Lee,3 and Yong Keun Park1,*

Graduate School of Biotechnology, Korea University, Seoul 136-701,1 Department of Biology, Hanseo University, Seosan 356-706,2 and Department of Microbiology, Hannam University, DaeJeon 300-791,3 Korea

Received 28 February 2001/Accepted 29 June 2001

Both Salmonella enterica serovar Typhimurium and Escherichia coli contain the cspH gene encoding CspH, one of the cold shock proteins (CSPs). In this study, we investigated the expression of cspH in S. enterica serovar Typhimurium and found that it was induced in response to a temperature downshift during exponential phase. The cspH promoter was activated at 37°C, and its mRNA was more stable than the other csp mRNAs at 37°C. Moreover, lacZ expression of the translational cspH-lacZ fusion was induced at that temperature. Interestingly, the cspH mRNA had a much shorter 5'-untranslated region than those in the other cold-shock-inducible genes, and the promoter sequence, which was only 55 bp, was sufficient for cspH expression. The 14-base downstream box located 12 bases downstream of the initiation codon of cspH mRNA was essential for its cold shock activation.

* Corresponding author. Mailing address: Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea. Phone: 82-2-3290-3422. Fax: 82-2-927-9028. E-mail: ykpark{at}mail.korea.ac.kr.

Journal of Bacteriology, October 2001, p. 5580-5588, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5580-5588.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Phadtare, S., Severinov, K. (2005). Extended -10 Motif Is Critical for Activity of the cspA Promoter but Does Not Contribute to Low-Temperature Transcription. J. Bacteriol. 187: 6584-6589 [Abstract] [Full Text]  
  • Jager, S., Evguenieva-Hackenberg, E., Klug, G. (2004). Temperature-dependent processing of the cspA mRNA in Rhodobacter capsulatus. Microbiology 150: 687-695 [Abstract] [Full Text]  


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