This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Borremans, B.
Right arrow Articles by van der Lelie, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Borremans, B.
Right arrow Articles by van der Lelie, D.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2001, p. 5651-5658, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5651-5658.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cloning and Functional Analysis of the pbr Lead Resistance Determinant of Ralstonia metallidurans CH34

B. Borremans,1 J. L. Hobman,2 A. Provoost,1 N. L. Brown,2 and D. van der Lelie1,*

VITO, Vlaamse Instelling voor Technologisch Onderzoek, Environmental Technology Centre, Boeretang 200, 2400 Mol, Belgium,1 and School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom2

Received 14 May 2001/Accepted 11 July 2001

The lead resistance operon, pbr, of Ralstonia metallidurans (formerly Alcaligenes eutrophus) strain CH34 is unique, as it combines functions involved in uptake, efflux, and accumulation of Pb(II). The pbr lead resistance locus contains the following structural resistance genes: (i) pbrT, which encodes a Pb(II) uptake protein; (ii) pbrA, which encodes a P-type Pb(II) efflux ATPase; (iii) pbrB, which encodes a predicted integral membrane protein of unknown function; and (iv) pbrC, which encodes a predicted prolipoprotein signal peptidase. Downstream of pbrC, the pbrD gene, encoding a Pb(II)-binding protein, was identified in a region of DNA, which was essential for functional lead sequestration. Pb(II)-dependent inducible transcription of pbrABCD from the PpbrA promoter is regulated by PbrR, which belongs to the MerR family of metal ion-sensing regulatory proteins. This is the first report of a mechanism for specific lead resistance in any bacterial genus.

* Corresponding author. Present address: Brookhaven National Laboratory (BNL), Biology Department, Upton, NY 11973-5000. Phone: (631) 344-5349. Fax: (631) 344-3407. E-mail: vdlelied{at}bnl.gov.

Journal of Bacteriology, October 2001, p. 5651-5658, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5651-5658.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Monchy, S., Benotmane, M. A., Janssen, P., Vallaeys, T., Taghavi, S., van der Lelie, D., Mergeay, M. (2007). Plasmids pMOL28 and pMOL30 of Cupriavidus metallidurans Are Specialized in the Maximal Viable Response to Heavy Metals. J. Bacteriol. 189: 7417-7425 [Abstract] [Full Text]  
  • Monchy, S., Benotmane, M. A., Wattiez, R., van Aelst, S., Auquier, V., Borremans, B., Mergeay, M., Taghavi, S., van der Lelie, D., Vallaeys, T. (2006). Transcriptomic and proteomic analyses of the pMOL30-encoded copper resistance in Cupriavidus metallidurans strain CH34. Microbiology 152: 1765-1776 [Abstract] [Full Text]  
  • Coombs, J. M., Barkay, T. (2005). New Findings on Evolution of Metal Homeostasis Genes: Evidence from Comparative Genome Analysis of Bacteria and Archaea. Appl. Environ. Microbiol. 71: 7083-7091 [Abstract] [Full Text]  
  • Coombs, J. M., Barkay, T. (2004). Molecular Evidence for the Evolution of Metal Homeostasis Genes by Lateral Gene Transfer in Bacteria from the Deep Terrestrial Subsurface. Appl. Environ. Microbiol. 70: 1698-1707 [Abstract] [Full Text]  
  • Mire, C. E., Tourjee, J. A., O'Brien, W. F., Ramanujachary, K. V., Hecht, G. B. (2004). Lead Precipitation by Vibrio harveyi: Evidence for Novel Quorum-Sensing Interactions. Appl. Environ. Microbiol. 70: 855-864 [Abstract] [Full Text]  
  • Broker, D., Arenskotter, M., Legatzki, A., Nies, D. H., Steinbuchel, A. (2004). Characterization of the 101-Kilobase-Pair Megaplasmid pKB1, Isolated from the Rubber-Degrading Bacterium Gordonia westfalica Kb1. J. Bacteriol. 186: 212-225 [Abstract] [Full Text]  
  • Gaballa, A., Cao, M., Helmann, J. D. (2003). Two MerR homologues that affect copper induction of the Bacillus subtilis copZA operon. Microbiology 149: 3413-3421 [Abstract] [Full Text]  
  • Legatzki, A., Grass, G., Anton, A., Rensing, C., Nies, D. H. (2003). Interplay of the Czc System and Two P-Type ATPases in Conferring Metal Resistance to Ralstonia metallidurans. J. Bacteriol. 185: 4354-4361 [Abstract] [Full Text]  
  • Weightman, A. J., Topping, A. W., Hill, K. E., Lee, L. L., Sakai, K., Slater, J. H., Thomas, A. W. (2002). Transposition of DEH, a Broad-Host-Range Transposon Flanked by ISPpu12, in Pseudomonas putida Is Associated with Genomic Rearrangements and Dehalogenase Gene Silencing. J. Bacteriol. 184: 6581-6591 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»