This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pronk, L. M. Articles by Sanderson, K. E. Search for Related Content PubMed PubMed Citation Articles by Pronk, L. M. Articles by Sanderson, K. E.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2001, p. 5782-5787, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5782-5787.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Intervening Sequences in rrl Genes and Fragmentation of 23S rRNA in Genera of the Family Enterobacteriaceae

Loes M. Pronk and Kenneth E. Sanderson^*

Salmonella Genetic Stock Centre, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4

Received 31 July 2000/Accepted 27 June 2001

Intervening sequences (IVSs) in the rrl genes for 23S rRNA are transcribed but later removed by RNase III without religation during RNA processing, leading to fragmented rRNA. We examined about 240 strains of the family Enterobacteriaceae for presence of IVSs using PCR. No IVSs were detected in strains belonging to Escherichia, Shigella, Enterobacter, Erwinia, Ewingella, Hafnia, Kluyvera, Morganella, Pantoea, or Serratia. Previously unreported IVSs were detected in Klebsiella oxytoca, Citrobacter amalonaticus, and Providencia stuartii; previously reported IVSs are in species of Salmonella, Proteus, Providencia, and Yersinia. The sporadic distribution of IVSs indicates lateral genetic transfer of IVSs.

---

^* Corresponding author. Mailing address: Salmonella Genetic Stock Centre, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4. Phone: (403) 220-6792 or (403) 220-3572. Fax: (403) 289-9311. E-mail: kesander{at}ucalgary.ca.

Present address: Syngenta Seeds BV, afd. Seedtechnology, 1606 BK Enkhuizen, The Netherlands.

---

Journal of Bacteriology, October 2001, p. 5782-5787, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5782-5787.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Chan, K., Miller, W. G., Mandrell, R. E., Kathariou, S. (2007). The Absence of Intervening Sequences in 23S rRNA Genes of Campylobacter coli Isolates from Turkeys Is a Unique Attribute of a Cluster of Related Strains Which Also Lack Resistance to Erythromycin. Appl. Environ. Microbiol. 73: 1208-1214 [Abstract] [Full Text]  
• Jones, A. M., Goodwill, A., Elliott, T. (2006). Limited Role for the DsrA and RprA Regulatory RNAs in rpoS Regulation in Salmonella enterica.. J. Bacteriol. 188: 5077-5088 [Abstract] [Full Text]  
• Crapoulet, N., Robineau, S., Raoult, D., Renesto, P. (2005). Intervening Sequence Acquired by Lateral Gene Transfer in Tropheryma whipplei Results in 23S rRNA Fragmentation. Appl. Environ. Microbiol. 71: 6698-6701 [Abstract] [Full Text]  
• Dewhirst, F. E., Shen, Z., Scimeca, M. S., Stokes, L. N., Boumenna, T., Chen, T., Paster, B. J., Fox, J. G. (2005). Discordant 16S and 23S rRNA Gene Phylogenies for the Genus Helicobacter: Implications for Phylogenetic Inference and Systematics. J. Bacteriol. 187: 6106-6118 [Abstract] [Full Text]  
• Baker, B. J., Hugenholtz, P., Dawson, S. C., Banfield, J. F. (2003). Extremely Acidophilic Protists from Acid Mine Drainage Host Rickettsiales-Lineage Endosymbionts That Have Intervening Sequences in Their 16S rRNA Genes. Appl. Environ. Microbiol. 69: 5512-5518 [Abstract] [Full Text]  
• Naderer, M., Brust, J. R., Knowle, D., Blumenthal, R. M. (2002). Mobility of a Restriction-Modification System Revealed by Its Genetic Contexts in Three Hosts. J. Bacteriol. 184: 2411-2419 [Abstract] [Full Text]