This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adams, M. W. W.
Right arrow Articles by Verhagen, M. F. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adams, M. W. W.
Right arrow Articles by Verhagen, M. F. J. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2001, p. 716-724, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.716-724.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Key Role for Sulfur in Peptide Metabolism and in Regulation of Three Hydrogenases in the Hyperthermophilic Archaeon Pyrococcus furiosus

Michael W. W. Adams,1,* James F. Holden,1 Angeli Lal Menon,1 Gerrit J. Schut,1 Amy M. Grunden,1,dagger Chun Hou,2 Andrea M. Hutchins,1 Francis E. Jenney Jr.,1 Chulhwan Kim,1 Kesen Ma,1,Dagger Guangliang Pan,1 Roopali Roy,1 Rajat Sapra,1 Sherry V. Story,1 and Marc F. J. M. Verhagen1,§

Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602-7229,1 and Department of Biology, Yunnan University, Kunming 650091, People's Republic of China2

Received 7 June 2000/Accepted 25 October 2000

The hyperthermophilic archaeon Pyrococcus furiosus grows optimally at 100°C by the fermentation of peptides and carbohydrates. Growth of the organism was examined in media containing either maltose, peptides (hydrolyzed casein), or both as the carbon source(s), each with and without elemental sulfur (S0). Growth rates were highest on media containing peptides and S0, with or without maltose. Growth did not occur on the peptide medium without S0. S0 had no effect on growth rates in the maltose medium in the absence of peptides. Phenylacetate production rates (from phenylalanine fermentation) from cells grown in the peptide medium containing S0 with or without maltose were the same, suggesting that S0 is required for peptide utilization. The activities of 14 of 21 enzymes involved in or related to the fermentation pathways of P. furiosus were shown to be regulated under the five different growth conditions studied. The presence of S0 in the growth media resulted in decreases in specific activities of two cytoplasmic hydrogenases (I and II) and of a membrane-bound hydrogenase, each by an order of magnitude. The primary S0-reducing enzyme in this organism and the mechanism of the S0 dependence of peptide metabolism are not known. This study provides the first evidence for a highly regulated fermentation-based metabolism in P. furiosus and a significant regulatory role for elemental sulfur or its metabolites.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Life Sciences Building, University of Georgia, Athens, GA 30602. Phone: (706) 542-2060. Fax: (706) 542-0229. E-mail: adams{at}bmb.uga.edu.

dagger Present address: Department of Microbiology, North Carolina State University, Raleigh, NC 27695.

Dagger Present address: Department of Biology, University of Waterloo, Waterloo, Ontario, N2L 3G1 Canada.

§ Present address: Allergan Inc., Irvine, CA 92612.

Journal of Bacteriology, January 2001, p. 716-724, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.716-724.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mardanov, A. V., Ravin, N. V., Svetlitchnyi, V. A., Beletsky, A. V., Miroshnichenko, M. L., Bonch-Osmolovskaya, E. A., Skryabin, K. G. (2009). Metabolic Versatility and Indigenous Origin of the Archaeon Thermococcus sibiricus, Isolated from a Siberian Oil Reservoir, as Revealed by Genome Analysis. Appl. Environ. Microbiol. 75: 4580-4588 [Abstract] [Full Text]  
  • Anderson, I., Rodriguez, J., Susanti, D., Porat, I., Reich, C., Ulrich, L. E., Elkins, J. G., Mavromatis, K., Lykidis, A., Kim, E., Thompson, L. S., Nolan, M., Land, M., Copeland, A., Lapidus, A., Lucas, S., Detter, C., Zhulin, I. B., Olsen, G. J., Whitman, W., Mukhopadhyay, B., Bristow, J., Kyrpides, N. (2008). Genome Sequence of Thermofilum pendens Reveals an Exceptional Loss of Biosynthetic Pathways without Genome Reduction. J. Bacteriol. 190: 2957-2965 [Abstract] [Full Text]  
  • van Haaster, D. J., Silva, P. J., Hagedoorn, P.-L., Jongejan, J. A., Hagen, W. R. (2008). Reinvestigation of the Steady-State Kinetics and Physiological Function of the Soluble NiFe-Hydrogenase I of Pyrococcus furiosus. J. Bacteriol. 190: 1584-1587 [Abstract] [Full Text]  
  • Feinberg, L. F., Srikanth, R., Vachet, R. W., Holden, J. F. (2008). Constraints on Anaerobic Respiration in the Hyperthermophilic Archaea Pyrobaculum islandicum and Pyrobaculum aerophilum. Appl. Environ. Microbiol. 74: 396-402 [Abstract] [Full Text]  
  • Chou, C.-J., Shockley, K. R., Conners, S. B., Lewis, D. L., Comfort, D. A., Adams, M. W. W., Kelly, R. M. (2007). Impact of Substrate Glycoside Linkage and Elemental Sulfur on Bioenergetics of and Hydrogen Production by the Hyperthermophilic Archaeon Pyrococcus furiosus. Appl. Environ. Microbiol. 73: 6842-6853 [Abstract] [Full Text]  
  • Schut, G. J., Bridger, S. L., Adams, M. W. W. (2007). Insights into the Metabolism of Elemental Sulfur by the Hyperthermophilic Archaeon Pyrococcus furiosus: Characterization of a Coenzyme A- Dependent NAD(P)H Sulfur Oxidoreductase. J. Bacteriol. 189: 4431-4441 [Abstract] [Full Text]  
  • Feinberg, L. F., Holden, J. F. (2006). Characterization of Dissimilatory Fe(III) versus NO3- Reduction in the Hyperthermophilic Archaeon Pyrobaculum aerophilum. J. Bacteriol. 188: 525-531 [Abstract] [Full Text]  
  • Fukui, T., Atomi, H., Kanai, T., Matsumi, R., Fujiwara, S., Imanaka, T. (2005). Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes. Genome Res 15: 352-363 [Abstract] [Full Text]  
  • Weinberg, M. V., Schut, G. J., Brehm, S., Datta, S., Adams, M. W. W. (2005). Cold Shock of a Hyperthermophilic Archaeon: Pyrococcus furiosus Exhibits Multiple Responses to a Suboptimal Growth Temperature with a Key Role for Membrane-Bound Glycoproteins. J. Bacteriol. 187: 336-348 [Abstract] [Full Text]  
  • Laska, S., Lottspeich, F., Kletzin, A. (2003). Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens. Microbiology 149: 2357-2371 [Abstract] [Full Text]  
  • Schut, G. J., Brehm, S. D., Datta, S., Adams, M. W. W. (2003). Whole-Genome DNA Microarray Analysis of a Hyperthermophile and an Archaeon: Pyrococcus furiosus Grown on Carbohydrates or Peptides. J. Bacteriol. 185: 3935-3947 [Abstract] [Full Text]  
  • Gao, J., Bauer, M. W., Shockley, K. R., Pysz, M. A., Kelly, R. M. (2003). Growth of Hyperthermophilic Archaeon Pyrococcus furiosus on Chitin Involves Two Family 18 Chitinases. Appl. Environ. Microbiol. 69: 3119-3128 [Abstract] [Full Text]  
  • Eisen, J. A., Nelson, K. E., Paulsen, I. T., Heidelberg, J. F., Wu, M., Dodson, R. J., Deboy, R., Gwinn, M. L., Nelson, W. C., Haft, D. H., Hickey, E. K., Peterson, J. D., Durkin, A. S., Kolonay, J. L., Yang, F., Holt, I., Umayam, L. A., Mason, T., Brenner, M., Shea, T. P., Parksey, D., Nierman, W. C., Feldblyum, T. V., Hansen, C. L., Craven, M. B., Radune, D., Vamathevan, J., Khouri, H., White, O., Gruber, T. M., Ketchum, K. A., Venter, J. C., Tettelin, H., Bryant, D. A., Fraser, C. M. (2002). The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium. Proc. Natl. Acad. Sci. USA 99: 9509-9514 [Abstract] [Full Text]  
  • Klein, R. J., Misulovin, Z., Eddy, S. R. (2002). Noncoding RNA genes identified in AT-rich hyperthermophiles. Proc. Natl. Acad. Sci. USA 99: 7542-7547 [Abstract] [Full Text]  
  • Andersson, J. O., Roger, A. J. (2002). Evolutionary Analyses of the Small Subunit of Glutamate Synthase: Gene Order Conservation, Gene Fusions, and Prokaryote-to- Eukaryote Lateral Gene Transfers. Eukaryot Cell 1: 304-310 [Abstract] [Full Text]  
  • Musfeldt, M., Schonheit, P. (2002). Novel Type of ADP-Forming Acetyl Coenzyme A Synthetase in Hyperthermophilic Archaea: Heterologous Expression and Characterization of Isoenzymes from the Sulfate Reducer Archaeoglobus fulgidus and the Methanogen Methanococcus jannaschii. J. Bacteriol. 184: 636-644 [Abstract] [Full Text]  
  • Schut, G. J., Zhou, J., Adams, M. W. W. (2001). DNA Microarray Analysis of the Hyperthermophilic Archaeon Pyrococcus furiosus: Evidence for a New Type of Sulfur-Reducing Enzyme Complex. J. Bacteriol. 183: 7027-7036 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»