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Journal of Bacteriology, January 2001, p. 736-744, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.736-744.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Clonal Population Structure of Pseudomonas stutzeri, a Species with Exceptional Genetic Diversity

Núria Rius,1 M. Carme Fusté,1 Caterina Guasp,2 Jorge Lalucat,2 and José G. Lorén1,*

Departament de Microbiologia i Parasitologia Sanitàries, Divisió de Ciències de la Salut, Universitat de Barcelona, 08028 Barcelona,1 and Departament de Biologia Ambiental, Universitat de les Illes Balears and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), 07071 Palma de Mallorca,2 Spain

Received 20 April 2000/Accepted 16 October 2000

Genetic diversity and genetic relationships among 42 Pseudomonas stutzeri strains belonging to several genomovars and isolated from different sources were investigated in an examination of 20 metabolic enzymes by multilocus enzyme electrophoresis analysis. Forty-two distinct allele profiles were identified, indicating that all multilocus genotypes were represented by a single strain. All 20 loci were exceptionally polymorphic, with an average of 15.9 alleles per locus. To the best of our knowledge, this P. stutzeri sample exhibited the highest mean genetic diversity (H = 0.876) found to date in all bacterial species studied by multilocus enzyme electrophoresis. A high frequency of occurrence of null alleles was identified. The index of association (IA) for the P. stutzeri strains analyzed was 1.10. The IA values were always significantly different from zero for all subgroups studied, including clinical and environmental isolates and strains classified as genomovar 1. These results suggest that the population structure of P. stutzeri is strongly clonal, indicating that there is no significant level of assortative recombination that might destroy linkage disequilibrium.

* Corresponding author. Mailing address: Laboratori de Microbiologia Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain. Phone: 34-93-402.4497. Fax: 34-93-402.1896. E-mail: loren{at}farmacia.far.ub.es.

Journal of Bacteriology, January 2001, p. 736-744, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.736-744.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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