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Journal of Bacteriology, January 2001, p. 752-757, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.752-757.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Initiation of Anaerobic Degradation of p-Cresol by Formation of 4-Hydroxybenzylsuccinate in Desulfobacterium cetonicum

Jochen A. Müller,* Alexander S. Galushko, Andreas Kappler, and Bernhard Schink

Fakultät für Biologie, Universität Konstanz, 78457 Konstanz, Germany

Received 13 April 2000/Accepted 6 October 2000

The anaerobic bacterium Desulfobacterium cetonicum oxidized p-cresol completely to CO2 with sulfate as the electron acceptor. During growth, 4-hydroxybenzylsuccinate accumulated in the medium. This finding indicated that the methyl group of p-cresol is activated by addition to fumarate, analogous to anaerobic toluene, m-xylene, and m-cresol degradation. In cell extracts, the formation of 4-hydroxybenzylsuccinate from p-cresol and fumarate was detected at an initial rate of 0.57 nmol min-1 (mg of protein)-1. This activity was specific for extracts of p-cresol-grown cells. 4-Hydroxybenzylsuccinate was degraded further to 4-hydroxybenzoyl-coenzyme A (CoA), most likely via beta -oxidation. 4-Hydroxybenzoyl-CoA was reductively dehydroxylated to benzoyl-CoA. There was no evidence of degradation of p-cresol via methyl group oxidation by p-cresol-methylhydroxylase in this bacterium.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305-4020. Phone: (650) 723-0315. Fax: (650) 725-3162. E-mail: jmueller{at}stanford.edu.

Journal of Bacteriology, January 2001, p. 752-757, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.752-757.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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