This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ding, Y.-H. R.
Right arrow Articles by Morgan, H. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ding, Y.-H. R.
Right arrow Articles by Morgan, H. W.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2001, p. 791-794, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.791-794.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Thermotoga maritima Phosphofructokinases: Expression and Characterization of Two Unique Enzymes

Yan-Huai R. Ding, Ron S. Ronimus, and Hugh W. Morgan*

Thermophile Research Unit, Department of Biological Sciences, The University of Waikato, Hamilton, New Zealand

Received 5 June 2000/Accepted 25 October 2000

A pyrophosphate-dependent phosphofructokinase (PPi-PFK) and an ATP-dependent phosphofructokinase (ATP-PFK) from Thermotoga maritima have been cloned and characterized. The PPi-PFK is unique in that the Km and Vmax values indicate that polyphosphate is the preferred substrate over pyrophosphate; the enzyme in reality is a polyphosphate-dependent PFK. The ATP-PFK was not significantly affected by common allosteric effectors (e.g., phosphoenolpyruvate) but was strongly inhibited by PPi and polyphosphate. The results suggest that the control of the Embden-Meyerhof pathway in this organism is likely to be modulated by pyrophosphate and/or polyphosphate.

* Corresponding author. Mailing address: Thermophile Research Unit, The University of Waikato, Private Bag 3105, Hamilton, New Zealand. Phone: 64-7-8388266. Fax: 64-7-8384324. E-mail: h.morgan{at}waikato.ac.nz.

Journal of Bacteriology, January 2001, p. 791-794, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.791-794.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Muller, M., Lee, J. A., Gordon, P., Gaasterland, T., Sensen, C. W. (2001). Presence of Prokaryotic and Eukaryotic Species in All Subgroups of the PPi-Dependent Group II Phosphofructokinase Protein Family. J. Bacteriol. 183: 6714-6716 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»