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Journal of Bacteriology, January 2001, p. 795-799, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.795-799.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of the Promoter of PRS1 in Saccharomyces cerevisiae Identifies Three Regions Potentially Involved in Control of Expression

Yolanda Hernando,1 Andrew T. Carter,1 Stefan Sickinger,2 and Michael Schweizer3,*

Department of Biological Sciences, Heriot-Watt University, Edinburgh, EH14 4AS,3 and Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA,1 United Kingdom, and Experimental Rheumatology Unit, Friedrich-Schiller Universität, D-07745, Jena, Germany2

Received 31 August 2000/Accepted 31 October 2000

The transcription initiation site of the Saccharomyces cerevisiae PRS1 gene was mapped at -179 bp. Measurement of beta -galactosidase activity of the successively deleted PRS1 promoter linked to lacZ and integrated at the ura3 locus defined three DNA regions involved in the control of PRS1 expression. Gel shift analysis confirmed the data.

* Corresponding author. Mailing address: Department of Biological Sciences, Heriot-Watt University, Edinburgh, EH14 4AS Scotland, United Kingdom. Phone: 44 131 451 3186 or 3182. Fax: 44 131 451 3009. E-mail: M.Schweizer{at}hw.ac.uk.

Journal of Bacteriology, January 2001, p. 795-799, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.795-799.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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