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Journal of Bacteriology, October 2001, p. 5877-5884, Vol. 183, No. 20
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.20.5877-5884.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Involvement of Two Distinct Catabolite-Responsive
Elements in Catabolite Repression of the Bacillus subtilis
myo-Inositol (iol) Operon
Yasuhiko
Miwa1 and
Yasutaro
Fujita2,*
Departments of Marine
Biotechnology1 and
Biotechnology,2 Faculty of
Engineering, Fukuyama University, Fukuyama 729-0292, Japan
Received 29 May 2001/Accepted 13 July 2001
The Bacillus subtilis inositol operon
(iolABCDEFGHIJ) is involved in
myo-inositol catabolism. Glucose repression of the
iol operon induced by inositol is exerted through
catabolite repression mediated by CcpA and the iol
induction system mediated by IolR. In this study, we identified two
iol catabolite-responsive elements (cre's), to which CcpA complexed with P-Ser-HPr or
P-Ser-Crh probably binds. One is located in iolB
(cre-iolB, nucleotides +2397 to +2411; +1
is the transcription initiation nucleotide), which was the only
cre-iol found in the previous
cre search of the B. subtilis genome
using a query sequence of WTGNAANCGNWNNCW (W stands for A or
T, and N stands for any base). Deletion and base substitution analysis
of the iol region indicated that
cre-iolB functions even if it is located
far downstream of the iol promoter. Further deletion and
base substitution analysis revealed another cre located
between the iol promoter and the iolA
gene (cre-iiolA, nucleotides +86 to
+100); the prefix "i" indicates a location in the intergenic region. Both cre-iiolA and
cre-iolB appeared to be recognized to
almost the same extent by CcpA complexed with either P-Ser-HPr or
P-Ser-Crh. Sequence alignment of the six known cre's,
including cre-iiolA, which were not
revealed in the previous cre search, exhibited
another consensus sequence of WTGAAARCGYTTWWN (R stands for
A or G, and Y stands for C or T); the right two thymines (TT) were
found to be essential for the function of
cre-iiolA by means of base substitution
analysis. A cre search with this query sequence led to
the finding of 14 additional putative cre's.
*
Corresponding author. Mailing address: Department of
Biotechnology, Faculty of Engineering, Fukuyama University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan. Phone: 81 849 36 2111. Fax: 81 849 36 2459. E-mail:
yfujita{at}bt.fubt.fukuyama-u.ac.jp.
Journal of Bacteriology, October 2001, p. 5877-5884, Vol. 183, No. 20
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.20.5877-5884.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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