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Journal of Bacteriology, October 2001, p. 6065-6073, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6065-6073.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

RecA-Mediated Rescue of Escherichia coli Strains with Replication Forks Arrested at the Terminus

Sophie Maisnier-Patin, Kurt Nordström, and Santanu Dasgupta*

Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, S-751 24 Uppsala, Sweden

Received 23 May 2001/Accepted 20 July 2001

The recombinational rescue of chromosome replication was investigated in Escherichia coli strains with the unidirectional origin oriR1, from the plasmid R1, integrated within oriC in clockwise (intR1CW) or counterclockwise (intR1CC) orientations. Only the intR1CC strain, with replication forks arrested at the terminus, required RecA for survival. Unlike the strains with RecA-dependent replication known so far, the intR1CC strain did not require RecBCD, RecF, RecG, RecJ, RuvAB, or SOS activation for viability. The overall levels of degradation of replicating chromosomes caused by inactivation of RecA were similar in oriC and intR1CC strains. In the intR1CC strain, RecA was also needed to maintain the integrity of the chromosome when the unidirectional replication forks were blocked at the terminus. This was consistent with suppression of the RecA dependence of the intR1CC strain by inactivating Tus, the protein needed to block replication forks at Ter sites. Thus, RecA is essential during asymmetric chromosome replication for the stable maintenance of the forks arrested at the terminus and for their eventual passage across the termination barrier(s) independently of the SOS and some of the major recombination pathways.


* Corresponding author. Mailing address: Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Box 596, S-751 24 Uppsala, Sweden. Phone: 46 18 471 4527. Fax: 46 18 53 03 96. E-mail: santanu.dasgupta{at}icm.uu.se.


Journal of Bacteriology, October 2001, p. 6065-6073, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6065-6073.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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