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Journal of Bacteriology, October 2001, p. 6107-6118, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6107-6118.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cytochrome Complex Essential for Photosynthetic Oxidation of both Thiosulfate and Sulfide in Rhodovulum sulfidophilum

Corinne Appia-Ayme,1 Phillip J. Little,1 Yumi Matsumoto,2 Andrew P. Leech,1 and Ben C. Berks1,dagger ,*

Center for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom,1 and Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan2

Received 2 April 2001/Accepted 17 July 2001

Many photosynthetic bacteria use inorganic sulfur compounds as electron donors for carbon dioxide fixation. A thiosulfate-induced cytochrome c has been purified from the photosynthetic alpha -proteobacterium Rhodovulum sulfidophilum. This cytochrome c551 is a heterodimer of a diheme 30-kDa SoxA subunit and a monoheme 15-kDa SoxX subunit. The cytochrome c551 structural genes are part of an 11-gene sox locus. Sequence analysis suggests that the ligands to the heme iron in SoxX are a methionine and a histidine, while both SoxA hemes are predicted to have unusual cysteine-plus-histidine coordination. A soxA mutant strain is unable to grow photoautotrophically on or oxidize either thiosulfate or sulfide. Cytochrome c551 is thus essential for the metabolism of both these sulfur species. Periplasmic extracts of wild-type R. sulfidophilum exhibit thiosulfate:cytochrome c oxidoreductase activity. However, such activity can only be measured for a soxA mutant strain if the periplasmic extract is supplemented with purified cytochrome c551. Gene clusters similar to the R. sulfidophilum sox locus can be found in the genome of a green sulfur bacterium and in phylogenetically diverse nonphotosynthetic autotrophs.

* Corresponding author. Mailing address: School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom. Phone: 44(0)1603 592186. Fax: 44(0)1603 592250. E-mail: b.berks{at}uea.ac.uk.

dagger Present address: Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom.

Journal of Bacteriology, October 2001, p. 6107-6118, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6107-6118.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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