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Journal of Bacteriology, October 2001, p. 6144-6147, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6144-6147.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Escherichia coli Minicell Membranes Are Enriched in Cardiolipin

Cecile-Marie Koppelman,1 Tanneke Den Blaauwen,1 Marc C. Duursma,2 Ron M. A. Heeren,2 and Nanne Nanninga1,*

Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam,1 and FOM-Institute for Atomic and Molecular Physics,2 Amsterdam, The Netherlands

Received 8 June 2001/Accepted 20 July 2001

The phospholipid composition of Escherichia coli minicells has been studied as a model for the cell division site. Minicells appeared to be enriched in cardiolipin at the expense of phosphatidylglycerol. Mass spectrometry showed no differences between the gross acyl chain compositions of minicells and wild-type cells.

* Corresponding author. Mailing address: Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands. Phone: 31-20-525 5194. Fax: 31-20-525 6271. E-mail: nanninga{at}science.uva.nl.

Journal of Bacteriology, October 2001, p. 6144-6147, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6144-6147.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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