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Journal of Bacteriology, November 2001, p. 6215-6224, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6215-6224.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification and Characterization of Tn4656, a Novel Class II Transposon Carrying a Set of Toluene-Degrading Genes from TOL Plasmid pWW53

Masataka Tsuda* and Hiroyuki Genka

Department of Environmental Life Science, Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Sendai 980-8577, Japan

Received 2 February 2001/Accepted 28 July 2001

It has been reported that the toluene-degrading (xyl) genes from Pseudomonas putida plasmid pWW53 are able to translocate to broad-host-range drug resistance plasmid RP4, and pWW53-4 is one of the smallest RP4 derivatives (H. Keil, S. Keil, R. W. Pickup, and P. A. Williams, J. Bacteriol. 164:887-895, 1985). Our investigation of pWW53-4 in this study demonstrated that such a translocated region that is 39 kb long is a transposon. This mobile element, Tn4656, was classified as a class II transposon since its transposition occurred by a two-step process: transposase (TnpA)-mediated formation of the cointegrate and resolvase (TnpR)-mediated site-specific resolution of the cointegrate at the two copies of the res site. The Tn4656 TnpA and TnpR functions encoded in the rightmost 4-kb region were found to be exchangeable with those specified by other Tn1721-related class II transposons, including another toluene transposon, Tn4653. Sequence analysis of the transposition-related genes and sites of Tn4656 also supported the hypothesis that this transposon is closely related to the Tn1721-related transposons. The lower transposition frequency of Tn4656 has been suggested to be due to the unique nucleotide sequence of one of the terminal 39-bp inverted repeats.

* Corresponding author. Mailing address: Department of Environmental Life Science, Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Sendai 980-8577, Japan. Phone: 81-22-217-5699. Fax: 81-22-217-5699. E-mail: mtsuda{at}ige.tohoku.ac.jp.

Journal of Bacteriology, November 2001, p. 6215-6224, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6215-6224.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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