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Journal of Bacteriology, November 2001, p. 6305-6314, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6305-6314.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Contributions of UP Elements and the Transcription Factor FIS to Expression from the Seven rrn P1 Promoters in Escherichia coli

Christine A. Hirvonen, Wilma Ross, Christopher E. Wozniak, Erin Marasco, Jennifer R. Anthony, Sarah E. Aiyar, Vanessa H. Newburn, and Richard L. Gourse*

Department of Bacteriology, University of Wisconsin---Madison, Madison, Wisconsin 53706

Received 18 June 2001/Accepted 2 August 2001

The high activity of the rrnB P1 promoter in Escherichia coli results from a cis-acting DNA sequence, the UP element, and a trans-acting transcription factor, FIS. In this study, we examine the effects of FIS and the UP element at the other six rrn P1 promoters. We find that UP elements are present at all of the rrn P1 promoters, but they make different relative contributions to promoter activity. Similarly, FIS binds upstream of, and activates, all seven rrn P1 promoters but to different extents. The total number of FIS binding sites, as well as their positions relative to the transcription start site, differ at each rrn P1 promoter. Surprisingly, the FIS sites upstream of site I play a much larger role in transcription from most rrn P1 promoters compared to rrnB P1. Our studies indicate that the overall activities of the seven rrn P1 promoters are similar, and the same contributors are responsible for these high activities, but these inputs make different relative contributions and may act through slightly different mechanisms at each promoter. These studies have implications for the control of gene expression of unlinked multigene families.

* Corresponding author. Mailing address: Department of Bacteriology, University of Wisconsin, 1550 Linden Dr., Madison WI 53706. Phone: (608) 262-9813. Fax: (608) 262-9865. E-mail: rgourse{at}bact.wisc.edu.

Journal of Bacteriology, November 2001, p. 6305-6314, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6305-6314.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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