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Journal of Bacteriology, November 2001, p. 6305-6314, Vol. 183, No. 21
Department of Bacteriology, University of
Wisconsin
Received 18 June 2001/Accepted 2 August 2001
The high activity of the rrnB P1 promoter in
Escherichia coli results from a
cis-acting DNA sequence, the UP element, and a
trans-acting transcription factor, FIS. In this study,
we examine the effects of FIS and the UP element at the other six
rrn P1 promoters. We find that UP elements are present
at all of the rrn P1 promoters, but they make different
relative contributions to promoter activity. Similarly, FIS binds
upstream of, and activates, all seven rrn P1 promoters
but to different extents. The total number of FIS binding sites, as
well as their positions relative to the transcription start site,
differ at each rrn P1 promoter. Surprisingly, the FIS
sites upstream of site I play a much larger role in transcription from
most rrn P1 promoters compared to rrnB P1. Our studies indicate that the overall activities of the seven rrn P1 promoters are similar, and the same contributors
are responsible for these high activities, but these inputs make
different relative contributions and may act through slightly different
mechanisms at each promoter. These studies have implications for the
control of gene expression of unlinked multigene families.
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.21.6305-6314.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Contributions of UP Elements and the Transcription Factor FIS to
Expression from the Seven rrn P1 Promoters in
Escherichia coli
Madison, Madison, Wisconsin 53706
*
Corresponding author. Mailing address: Department of
Bacteriology, University of Wisconsin, 1550 Linden Dr., Madison WI
53706. Phone: (608) 262-9813. Fax: (608) 262-9865. E-mail:
rgourse{at}bact.wisc.edu.
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