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Journal of Bacteriology, November 2001, p. 6491-6493, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6491-6493.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transcription-Induced Cytosine-to-Thymine Mutations Are Not Dependent on Sequence Context of the Target Cytosine

Anton Beletskiidagger and Ashok S. Bhagwat*

Department of Chemistry, Wayne State University, Detroit, Michigan 48202

Received 29 May 2001/Accepted 1 August 2001

We showed previously that transcription of a plasmid-borne kan allele increases C-to-T mutations in the nontranscribed strand. Using two new plasmid-borne kan alleles, one cmp allele, and a chromosomal kan allele, we found in this study that transcription-induced mutations are not limited to specific genes, alleles, or locations and are likely to be a general property of transcript elongation in Escherichia coli.

* Corresponding author. Mailing address: 463 Chemistry, Wayne State University, Detroit, MI 48202. Phone: (313) 577-2547. Fax: (313) 577-8822. E-mail: axb{at}chem.wayne.edu.

dagger Present address: Harvard Institute of Human Genetics, Boston, MA 02115.

Journal of Bacteriology, November 2001, p. 6491-6493, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6491-6493.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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