This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pichoff, S.
Right arrow Articles by Lutkenhaus, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pichoff, S.
Right arrow Articles by Lutkenhaus, J.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2001, p. 6630-6635, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6630-6635.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Escherichia coli Division Inhibitor MinCD Blocks Septation by Preventing Z-Ring Formation

Sebastien Pichoff and Joe Lutkenhaus*

Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160

Received 25 May 2001/Accepted 29 August 2001

The min system spatially regulates division through the topological regulation of MinCD, an inhibitor of cell division. MinCD was previously shown to inhibit division by preventing assembly of the Z ring (E. Bi and J. Lutkenhaus, J. Bacteriol. 175:1118-1125, 1993); however, this was questioned in a recent report (S. S. Justice, J. Garcia-Lara, and L. I. Rothfield, Mol. Microbiol. 37:410-423, 2000) which indicated that MinCD acted after Z-ring formation and prevented the recruitment of FtsA to the Z ring. This discrepancy was due in part to alternative fixation conditions. We have therefore reinvestigated the action of MinCD and avoided fixation by using green fluorescent protein (GFP) fusions to division proteins. MinCD prevented the localization of both FtsZ-GFP and ZipA-GFP, consistent with it preventing Z-ring assembly. Consistent with a direct interaction between FtsZ and the MinCD inhibitor, we find that increased FtsZ, but not FtsA, suppresses MinCD-induced lethality. Furthermore, strains carrying various alleles of ftsZ, selected on the basis of resistance to the inhibitor SulA, displayed variable resistance to MinCD. These results are consistent with FtsZ as the target of MinCD and confirm that this inhibitor prevents Z-ring assembly.

* Corresponding author. Mailing address: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center; Kansas City, KS 66160. Phone: (913) 588-7054. Fax: (913) 588-7295. E-mail: jlutkenh{at}kumc.edu.

Journal of Bacteriology, November 2001, p. 6630-6635, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6630-6635.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Oliferenko, S., Chew, T. G., Balasubramanian, M. K. (2009). Positioning cytokinesis. Genes Dev. 23: 660-674 [Abstract] [Full Text]  
  • Gregory, J. A., Becker, E. C., Pogliano, K. (2008). Bacillus subtilis MinC destabilizes FtsZ-rings at new cell poles and contributes to the timing of cell division. Genes Dev. 22: 3475-3488 [Abstract] [Full Text]  
  • Corbin, B. D., Wang, Y., Beuria, T. K., Margolin, W. (2007). Interaction between Cell Division Proteins FtsE and FtsZ. J. Bacteriol. 189: 3026-3035 [Abstract] [Full Text]  
  • Geissler, B., Shiomi, D., Margolin, W. (2007). The ftsA* gain-of-function allele of Escherichia coli and its effects on the stability and dynamics of the Z ring. Microbiology 153: 814-825 [Abstract] [Full Text]  
  • Castanie-Cornet, M.-P., Cam, K., Jacq, A. (2006). RcsF Is an Outer Membrane Lipoprotein Involved in the RcsCDB Phosphorelay Signaling Pathway in Escherichia coli.. J. Bacteriol. 188: 4264-4270 [Abstract] [Full Text]  
  • Aldridge, C., Moller, S. G. (2005). The Plastid Division Protein AtMinD1 Is a Ca2+-ATPase Stimulated by AtMinE1. J. Biol. Chem. 280: 31673-31678 [Abstract] [Full Text]  
  • Zhou, H., Lutkenhaus, J. (2005). MinC Mutants Deficient in MinD- and DicB-Mediated Cell Division Inhibition Due to Loss of Interaction with MinD, DicB, or a Septal Component. J. Bacteriol. 187: 2846-2857 [Abstract] [Full Text]  
  • Aldridge, C., Maple, J., Moller, S. G. (2005). The molecular biology of plastid division in higher plants. J Exp Bot 56: 1061-1077 [Abstract] [Full Text]  
  • Varma, A., Young, K. D. (2004). FtsZ Collaborates with Penicillin Binding Proteins To Generate Bacterial Cell Shape in Escherichia coli. J. Bacteriol. 186: 6768-6774 [Abstract] [Full Text]  
  • Johnson, J. E., Lackner, L. L., Hale, C. A., de Boer, P. A. J. (2004). ZipA Is Required for Targeting of DMinC/DicB, but Not DMinC/MinD, Complexes to Septal Ring Assemblies in Escherichia coli. J. Bacteriol. 186: 2418-2429 [Abstract] [Full Text]  
  • Huecas, S., Andreu, J. M. (2003). Energetics of the Cooperative Assembly of Cell Division Protein FtsZ and the Nucleotide Hydrolysis Switch. J. Biol. Chem. 278: 46146-46154 [Abstract] [Full Text]  
  • Zhou, H., Lutkenhaus, J. (2003). Membrane Binding by MinD Involves Insertion of Hydrophobic Residues within the C-Terminal Amphipathic Helix into the Bilayer. J. Bacteriol. 185: 4326-4335 [Abstract] [Full Text]  
  • Vitha, S., Froehlich, J. E., Koksharova, O., Pyke, K. A., van Erp, H., Osteryoung, K. W. (2003). ARC6 Is a J-Domain Plastid Division Protein and an Evolutionary Descendant of the Cyanobacterial Cell Division Protein Ftn2. Plant Cell 15: 1918-1933 [Abstract] [Full Text]  
  • Geissler, B., Elraheb, D., Margolin, W. (2003). A gain-of-function mutation in ftsA bypasses the requirement for the essential cell division gene zipA in Escherichia coli. Proc. Natl. Acad. Sci. USA 100: 4197-4202 [Abstract] [Full Text]  
  • Errington, J., Daniel, R. A., Scheffers, D.-J. (2003). Cytokinesis in Bacteria. Microbiol. Mol. Biol. Rev. 67: 52-65 [Abstract] [Full Text]  
  • Kroos, L., Maddock, J. R. (2003). Prokaryotic Development: Emerging Insights. J. Bacteriol. 185: 1128-1146 [Full Text]  
  • Lackner, L. L., Raskin, D. M., de Boer, P. A. J. (2003). ATP-Dependent Interactions between Escherichia coli Min Proteins and the Phospholipid Membrane In Vitro. J. Bacteriol. 185: 735-749 [Abstract] [Full Text]  
  • Hamoen, L. W., Errington, J. (2003). Polar Targeting of DivIVA in Bacillus subtilis Is Not Directly Dependent on FtsZ or PBP 2B. J. Bacteriol. 185: 693-697 [Abstract] [Full Text]  
  • Hu, Z., Saez, C., Lutkenhaus, J. (2003). Recruitment of MinC, an Inhibitor of Z-Ring Formation, to the Membrane in Escherichia coli: Role of MinD and MinE. J. Bacteriol. 185: 196-203 [Abstract] [Full Text]  
  • Suefuji, K., Valluzzi, R., RayChaudhuri, D. (2002). Dynamic assembly of MinD into filament bundles modulated by ATP, phospholipids, and MinE. Proc. Natl. Acad. Sci. USA 99: 16776-16781 [Abstract] [Full Text]  
  • Johnson, J. E., Lackner, L. L., de Boer, P. A. J. (2002). Targeting of DMinC/MinD and DMinC/DicB Complexes to Septal Rings in Escherichia coli Suggests a Multistep Mechanism for MinC-Mediated Destruction of Nascent FtsZ Rings. J. Bacteriol. 184: 2951-2962 [Abstract] [Full Text]  
  • Hu, Z., Gogol, E. P., Lutkenhaus, J. (2002). Dynamic assembly of MinD on phospholipid vesicles regulated by ATP and MinE. Proc. Natl. Acad. Sci. USA 99: 6761-6766 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»