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Journal of Bacteriology, November 2001, p. 6688-6693, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6688-6693.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Precise Deletion of tagD and Controlled Depletion of Its Product, Glycerol 3-Phosphate Cytidylyltransferase, Leads to Irregular Morphology and Lysis of Bacillus subtilis Grown at Physiological Temperature

Amit P. Bhavsar,1 Terry J. Beveridge,2 and Eric D. Brown1,*

Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5,1 and Department of Microbiology and Canadian Bacterial Disease Network, University of Guelph, Guelph, Ontario, Canada N1G 2W12

Received 25 June 2001/Accepted 28 August 2001

Using a previously reported conditional expression system for use in Bacillus subtilis (A. P. Bhavsar, X. Zhao, and E. D. Brown, Appl. Environ. Microbiol. 67:403-410, 2001), we report the first precise deletion of a teichoic acid biosynthesis (tag) gene, tagD, in B. subtilis. This teichoic acid mutant showed a lethal phenotype when characterized at a physiological temperature and in a defined genetic background. This tagD mutant was subject to full phenotypic rescue upon expression of the complementing copy of tagD. Depletion of the tagD gene product (glycerol 3-phosphate cytidylyltransferase) via modulated expression of tagD from the amyE locus revealed structural defects centered on shape, septation, and division. Thickening of the wall and ultimately lysis followed these events.

* Corresponding author. Mailing address: Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main St. West, Hamilton, Ontario L8N 3Z5, Canada. Phone: (905) 525-9140, ext. 22392. Fax: (905) 522-9033. E-mail: ebrown{at}mcmaster.ca.

Journal of Bacteriology, November 2001, p. 6688-6693, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6688-6693.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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