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Journal of Bacteriology, November 2001, p. 6707-6709, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6707-6709.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Hemin Reconstitutes Proton Extrusion in an H+-ATPase-Negative Mutant of Lactococcus lactis

Lars M. Blank,1 Brian J. Koebmann,2 Ole Michelsen,2 Lars K. Nielsen,1 and Peter R. Jensen2,*

Department of Chemical Engineering, University of Queensland, 4072 Brisbane, Australia,1 and Section of Molecular Microbiology, BioCentrum, Technical University of Denmark, DK-2800 Lyngby, Denmark2

Received 30 May 2001/Accepted 20 August 2001

H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.

* Corresponding author. Mailing address: Section of Molecular Microbiology, BioCentrum, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark. Phone: 45 45-252510. Fax: 45 45-932809. E-mail: Peter.R.Jensen{at}BioCentrum.DTU.DK.

Journal of Bacteriology, November 2001, p. 6707-6709, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6707-6709.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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