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 Previous Article

Journal of Bacteriology, November 2001, p. 6721-6725, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6721-6725.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Colicin A Immunity Protein Interacts with the Hydrophobic Helical Hairpin of the Colicin A Channel Domain in the Escherichia coli Inner Membrane

Angèle Nardi, Yves Corda, Daniel Baty, and Denis Duché*

Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Institut de Biologie Structurale et Microbiologie, CNRS, 13402 Marseille cedex 20, France

Received 5 March 2001/Accepted 23 August 2001

The colicin A pore-forming domain (pfColA) was fused to a bacterial signal peptide (sp-pfColA). This was inserted into the Escherichia coli inner membrane in functional form and could be coimmunoprecipitated with epitope-tagged immunity protein (EpCai). We constructed a series of fusion proteins in which various numbers of sp-pfColA alpha -helices were fused to alkaline phosphatase (AP). We showed that a fusion protein made up of the hydrophobic alpha -helices 8 and 9 of sp-pfColA fused to AP was specifically coimmunoprecipitated with EpCai produced in the same cells. This is the first biochemical evidence that Cai recognizes and interacts with the colicin A hydrophobic helical hairpin.

* Corresponding author. Mailing address: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Institut de Biologie Structurale et Microbiologie, CNRS, 31 chemin Joseph Aiguier, 13402 Marseille cedex 20, France. Phone: 33 04 91 16 45 61. Fax: 33 04 91 71 21 24. E-mail: duche{at}ibsm.cnrs-mrs.fr.

Journal of Bacteriology, November 2001, p. 6721-6725, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6721-6725.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cascales, E., Buchanan, S. K., Duche, D., Kleanthous, C., Lloubes, R., Postle, K., Riley, M., Slatin, S., Cavard, D. (2007). Colicin Biology. Microbiol. Mol. Biol. Rev. 71: 158-229 [Abstract] [Full Text]  
  • Naas, T., Zerbib, M., Girlich, D., Nordmann, P. (2003). Integration of a Transposon Tn1-Encoded Inhibitor-Resistant {beta}-Lactamase Gene, blaTEM-67 from Proteus mirabilis, into the Escherichia coli Chromosome. Antimicrob. Agents Chemother. 47: 19-26 [Abstract] [Full Text]  


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