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Journal of Bacteriology, December 2001, p. 6957-6960, Vol. 183, No. 23
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.23.6957-6960.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The rlmB Gene Is Essential for Formation of Gm2251 in 23S rRNA but Not for Ribosome Maturation in Escherichia coli

J. Mattias Lövgren and P. Mikael Wikström*

Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden

Received 9 May 2001/Accepted 14 September 2001

In Saccharomyces cerevisiae, the rRNA Gm2270 methyltransferase, Pet56p, has an essential role in the maturation of the mitochondrial large ribosomal subunit that is independent of its methyltransferase activity. Here we show that the proposed Escherichia coli ortholog, RlmB (formerly YjfH), indeed is essential for the formation of Gm in position 2251 of 23S rRNA. However, a Delta rlmB mutant did not show any ribosome assembly defects and was not outgrown by a wild-type strain even after 120 cell mass doublings. Thus, RlmB has no important role in ribosome assembly or function in E. coli.

* Corresponding author. Mailing address: Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden. Phone: 46-90-7856754. Fax: 46-90-772630. E-mail: Mikael.Wikstrom{at}micro.umu.se.

Journal of Bacteriology, December 2001, p. 6957-6960, Vol. 183, No. 23
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.23.6957-6960.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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