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Journal of Bacteriology, December 2001, p. 7087-7093, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7087-7093.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Involvement of hyp Gene Products in Maturation of the H2-Sensing [NiFe] Hydrogenase of Ralstonia eutropha

Thorsten Buhrke,1 Boris Bleijlevens,2 Simon P. J. Albracht,2 and Bärbel Friedrich1,*

Institut für Biologie, Humboldt-Universität zu Berlin, 10115 Berlin, Germany,1 and Swammerdam Institute for Life Sciences, Biochemistry, University of Amsterdam, NL-1018 TV Amsterdam, The Netherlands2

Received 23 May 2001/Accepted 17 September 2001

The biosynthesis of [NiFe] hydrogenases is a complex process that requires the function of the Hyp proteins HypA, HypB, HypC, HypD, HypE, HypF, and HypX for assembly of the H2-activating [NiFe] site. In this study we examined the maturation of the regulatory hydrogenase (RH) of Ralstonia eutropha. The RH is a H2-sensing [NiFe] hydrogenase and is required as a constituent of a signal transduction chain for the expression of two energy-linked [NiFe] hydrogenases. Here we demonstrate that the RH regulatory activity was barely affected by mutations in hypA, hypB, hypC, and hypX and was not substantially diminished in hypD- and hypE-deficient strains. The lack of HypF, however, resulted in a 90% decrease of the RH regulatory activity. Fourier transform infrared spectroscopy and the incorporation of 63Ni into the RH from overproducing cells revealed that the assembly of the [NiFe] active site is dependent on all Hyp functions, with the exception of HypX. We conclude that the entire Hyp apparatus (HypA, HypB, HypC, HypD, HypE, and HypF) is involved in an efficient incorporation of the [NiFe] center into the RH.

* Corresponding author. Mailing address: Institut für Biologie, Humboldt-Universität zu Berlin, Chausseestr. 117, 10115 Berlin, Germany. Phone: (49) 30-20-93-81-01. Fax: (49) 30-20-93-81-02. E-mail: baerbel.friedrich{at}rz.hu-berlin.de.

Journal of Bacteriology, December 2001, p. 7087-7093, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7087-7093.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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