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Journal of Bacteriology, December 2001, p. 7110-7119, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7110-7119.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transcriptional Analysis of the tet(P) Operon from Clostridium perfringens

Priscilla A. Johanesen, Dena Lyras, Trudi L. Bannam, and Julian I. Rood*

Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800, Australia

Received 28 June 2001/Accepted 26 September 2001

The Clostridium perfringens tetracycline resistance determinant from the 47-kb conjugative R-plasmid pCW3 is unique in that it consists of two overlapping genes, tetA(P) and tetB(P), which mediate resistance by different mechanisms. Detailed transcriptional analysis has shown that the inducible tetA(P) and tetB(P) genes comprise an operon that is transcribed from a single promoter, P3, located 529 bp upstream of the tetA(P) start codon. Deletion of P3 or alteration of the spacing between the -35 and -10 regions significantly reduced the level of transcription in a reporter construct. Induction was shown to be mediated at the level of transcription. Unexpectedly, a factor-independent terminator, T1, was detected downstream of P3 but before the start of the tetA(P) gene. Deletion or mutation of this terminator led to increased read-through transcription in the reporter construct. It is postulated that the T1 terminator is an intrinsic control element of the tet(P) operon and that it acts to prevent the overexpression of the TetA(P) transmembrane protein, even in the presence of tetracycline.

* Corresponding author. Mailing address: Department of Microbiology, P.O. Box 53, Monash University, Victoria 3800, Australia. Phone: 61 3 9905 4825. Fax: 61 3 9905 4811. E-mail: julian.rood{at}med.monash.edu.au.

Journal of Bacteriology, December 2001, p. 7110-7119, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7110-7119.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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