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Journal of Bacteriology, December 2001, p. 7145-7153, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7145-7153.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

ADP-Dependent Phosphofructokinases in Mesophilic and Thermophilic Methanogenic Archaea

Corné H. Verhees,* Judith E. Tuininga, Servé W. M. Kengen, Alfons J. M. Stams, John van der Oost, and Willem M. de Vos

Laboratory of Microbiology, Wageningen University, 6703 CT Wageningen, The Netherlands

Received 9 March 2001/Accepted 21 September 2001

Phosphofructokinase (PFK) is a key enzyme of the glycolytic pathway in all domains of life. Two related PFKs, ATP-dependent and PPi-dependent PFK, have been distinguished in bacteria and eucarya, as well as in some archaea. Hyperthermophilic archaea of the order Thermococcales, including Pyrococcus and Thermococcus spp., have recently been demonstrated to possess a unique ADP-dependent PFK (ADP-PFK) that appears to be phylogenetically distinct. Here, we report the presence of ADP-PFKs in glycogen-producing members of the orders Methanococcales and Methanosarcinales, including both mesophilic and thermophilic representatives. To verify the substrate specificities of the methanogenic kinases, the gene encoding the ADP-PFK from Methanococcus jannaschii was functionally expressed in Escherichia coli, and the produced enzyme was purified and characterized in detail. Compared to its counterparts from the two members of the order Thermococcales, the M. jannaschii ADP-PFK has an extremely low Km for fructose 6-phosphate (9.6 µM), and it accepts both ADP and acetyl-phosphate as phosphoryl donors. Phylogenetic analysis of the ADP-PFK reveals it to be a key enzyme of the modified Embden-Meyerhof pathway of heterotrophic and chemolithoautotrophic archaea. Interestingly, uncharacterized homologs of this unusual kinase are present in several eucarya.

* Corresponding author. Mailing address: Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. Phone: 31-317-483748. Fax: 31-317-483829. E-mail: corne.verhees{at}algemeen.micr.wau.nl.

Journal of Bacteriology, December 2001, p. 7145-7153, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7145-7153.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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