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Journal of Bacteriology, December 2001, p. 7145-7153, Vol. 183, No. 24
Laboratory of Microbiology, Wageningen
University, 6703 CT Wageningen, The Netherlands
Received 9 March 2001/Accepted 21 September 2001
Phosphofructokinase (PFK) is a key enzyme of the glycolytic pathway
in all domains of life. Two related PFKs, ATP-dependent and
PPi-dependent PFK, have been distinguished in bacteria and eucarya, as well as in some archaea. Hyperthermophilic archaea of the
order Thermococcales, including Pyrococcus and
Thermococcus spp., have recently been demonstrated to
possess a unique ADP-dependent PFK (ADP-PFK) that appears to be
phylogenetically distinct. Here, we report the presence of ADP-PFKs in
glycogen-producing members of the orders Methanococcales
and Methanosarcinales, including both mesophilic and
thermophilic representatives. To verify the substrate specificities of
the methanogenic kinases, the gene encoding the ADP-PFK from
Methanococcus jannaschii was functionally expressed in
Escherichia coli, and the produced enzyme was purified and
characterized in detail. Compared to its counterparts from the two
members of the order Thermococcales, the M. jannaschii ADP-PFK has an extremely low
Km for fructose 6-phosphate (9.6 µM), and it
accepts both ADP and acetyl-phosphate as phosphoryl donors.
Phylogenetic analysis of the ADP-PFK reveals it to be a key enzyme of
the modified Embden-Meyerhof pathway of heterotrophic and
chemolithoautotrophic archaea. Interestingly, uncharacterized homologs
of this unusual kinase are present in several eucarya.
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.24.7145-7153.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
ADP-Dependent Phosphofructokinases in Mesophilic
and Thermophilic Methanogenic Archaea
*
Corresponding author. Mailing address: Laboratory of
Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. Phone: 31-317-483748. Fax:
31-317-483829. E-mail:
corne.verhees{at}algemeen.micr.wau.nl.
Journal of Bacteriology, December 2001, p. 7145-7153, Vol. 183, No. 24
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.24.7145-7153.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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