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Journal of Bacteriology, December 2001, p. 7206-7212, Vol. 183, No. 24
Departamento de Genética Molecular,
Instituto de Fisiología Celular, Universidad Nacional
Autónoma de México, 04510 Mexico City,
Mexico1; Department of Microbiology and
Molecular Genetics, Harvard Medical School, Boston, Massachusetts
021152; and Department of Microbiology
and Immunology, University of Michigan Medical School, Ann Arbor,
Michigan 481093
Received 20 August 2001/Accepted 27 September 2001
The Arc (anoxic redox control) two-component signal transduction
system of Escherichia coli, which comprises the tripartite ArcB sensor kinase and the ArcA response regulator, modulates the
expression of numerous operons in response to redox conditions of
growth. We demonstrate that the arcA and arcB
genes of Haemophilus influenzae specify a two-component
system. The Arc proteins of the two bacterial species sufficiently
resemble each other that they can participate in heterologous
transphosphorylation in vitro. Moreover, the Arc system of H. influenzae mediates transcriptional control according to the
redox condition of growth both autologously in its own host and
homologously in E. coli, indicating a high degree of
functional conservation of the signal transduction system. The H. influenzae ArcB, however, lacks the PAS domain present in the
region of E. coli ArcB linking the transmembrane to the cytosolic catalytic domains. Because the PAS domain participates in
signal reception in a variety of sensory proteins, including sensors of
molecular oxygen and redox state, a similar role was previously
ascribed to it in ArcB. Our results demonstrate that the ArcB protein
of H. influenzae mediates signal transduction in response
to redox conditions of growth despite the absence of the PAS domain.
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.24.7206-7212.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Redox Signal Transduction by the ArcB Sensor Kinase
of Haemophilus influenzae Lacking the PAS Domain
*
Corresponding author. Mailing address: Department of
Microbiology and Immunology, University of Michigan Medical School,
1150 West Medical Ctr. Dr., Ann Arbor, MI 48109. Phone: (734) 615-4288. Fax: (734) 764-3562. E-mail: bakerley{at}umich.edu.
Journal of Bacteriology, December 2001, p. 7206-7212, Vol. 183, No. 24
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.24.7206-7212.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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