This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alves, A. M. C. R. Articles by Dijkhuizen, L. Search for Related Content PubMed PubMed Citation Articles by Alves, A. M. C. R. Articles by Dijkhuizen, L.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2001, p. 7231-7240, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7231-7240.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Different Physiological Roles of ATP- and PP_i-Dependent Phosphofructokinase Isoenzymes in the Methylotrophic Actinomycete Amycolatopsis methanolica

A. M. C. R. Alves,¹ G. J. W. Euverink,¹ H. Santos,² and L. Dijkhuizen^1,*

Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9751 NN Haren, The Netherlands,¹ and Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apartado 127 Oeiras, Portugal²

Received 16 April 2001/Accepted 18 September 2001

Cells of the actinomycete Amycolatopsis methanolica grown on glucose possess only a single, exclusively PP_i-dependent phosphofructokinase (PP_i-PFK) (A. M. C. R. Alves, G. J. W. Euverink, H. J. Hektor, J. van der Vlag, W. Vrijbloed, D.H.A. Hondmann, J. Visser, and L. Dijkhuizen, J. Bacteriol. 176:6827-6835, 1994). When this methylotrophic bacterium is grown on one-carbon (C₁) compounds (e.g., methanol), an ATP-dependent phosphofructokinase (ATP-PFK) activity is specifically induced, completely replacing the PP_i-PFK. The two A. methanolica PFK isoenzymes have very distinct functions, namely, in the metabolism of C₆ and C₁ carbon substrates. This is the first report providing biochemical evidence for the presence and physiological roles of PP_i-PFK and ATP-PFK isoenzymes in a bacterium. The novel ATP-PFK enzyme was purified to homogeneity and characterized in detail at the biochemical and molecular levels. The A. methanolica ATP-PFK and PP_i-PFK proteins possess a low level of amino acid sequence similarity (24%), clearly showing that the two proteins are not the result of a gene duplication event. PP_i-PFK is closely related to other (putative) actinomycete PFK enzymes. Surprisingly, the A. methanolica ATP-PFK is most similar to ATP-PFK from the protozoon Trypanosoma brucei and PP_i-PFK proteins from the bacteria Borrelia burgdorferi and Treponema pallidum, both spirochetes, very distinct from actinomycetes. The data thus suggest that A. methanolica obtained the ATP-PFK-encoding gene via a lateral gene transfer event.

---

^* Corresponding author. Mailing address: Department of Microbiology, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands. Phone: 31.50.3632150. Fax: 31.50.3632154. E-mail: L.Dijkhuizen{at}biol.rug.nl.

---

Journal of Bacteriology, December 2001, p. 7231-7240, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7231-7240.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Tanneberger, K., Kirchberger, J., Bar, J., Schellenberger, W., Rothemund, S., Kamprad, M., Otto, H., Schoneberg, T., Edelmann, A. (2007). A Novel Form of 6-Phosphofructokinase: IDENTIFICATION AND FUNCTIONAL RELEVANCE OF A THIRD TYPE OF SUBUNIT IN PICHIA PASTORIS. J. Biol. Chem. 282: 23687-23697 [Abstract] [Full Text]  
• Jakobsen, O. M., Benichou, A., Flickinger, M. C., Valla, S., Ellingsen, T. E., Brautaset, T. (2006). Upregulated Transcription of Plasmid and Chromosomal Ribulose Monophosphate Pathway Genes Is Critical for Methanol Assimilation Rate and Methanol Tolerance in the Methylotrophic Bacterium Bacillus methanolicus.. J. Bacteriol. 188: 3063-3072 [Abstract] [Full Text]  
• Brautaset, T., Jakobsen, O. M., Flickinger, M. C., Valla, S., Ellingsen, T. E. (2004). Plasmid-Dependent Methylotrophy in Thermotolerant Bacillus methanolicus. J. Bacteriol. 186: 1229-1238 [Abstract] [Full Text]