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Journal of Bacteriology, December 2001, p. 7253-7259, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7253-7259.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Clock Control of Ultradian Respiratory Oscillation Found during Yeast Continuous Culture

Douglas B. Murray,^*,1 Sibel Roller,¹ Hiroshi Kuriyama,² and David Lloyd³

School of Applied Science, South Bank University, London SE1 0AA,¹ and Microbiology (BIOSI), Cardiff University, Cardiff CF10 3TL,³ United Kingdom, and AIST Hokkaido Center, National Institute of Advanced Industrial Science and Technology, 2-17 Tukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan²

Received 21 May 2001/Accepted 18 September 2001

A short-period autonomous respiratory ultradian oscillation (period  40 min) occurs during aerobic Saccharomyces cerevisiae continuous culture and is most conveniently studied by monitoring dissolved O₂ concentrations. The resulting data are high quality and reveal fundamental information regarding cellular dynamics. The phase diagram and discrete fast Fourier transformation of the dissolved O₂ values revealed a square waveform with at least eight harmonic peaks. Stepwise changes in temperature revealed that the oscillation was temperature compensated at temperatures ranging from 27 to 34°C when either glucose (temperature quotient [Q₁₀] = 1.02) or ethanol (Q₁₀ = 0.82) was used as a carbon source. After alteration of the temperature beyond the temperature compensation region, phase coherence events for individual cells were quickly lost. As the cell doubling rate decreased from 15.5 to 9.2 h (a factor of 1.68), the periodicity decreased by a factor of 1.26. This indicated that there was a degree of nutrient compensation. Outside the range of dilution rates at which stable oscillation occurred, the mode of oscillation changed. The oscillation in respiratory output is therefore under clock control.

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^* Corresponding author. Mailing address: School of Applied Science, South Bank University, 103 Borough Road, London SE1 0AA, United Kingdom. Phone: 020 7815 7985. Fax: 020 7815 7999. E-mail: murraydb{at}sbu.ac.uk.

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Journal of Bacteriology, December 2001, p. 7253-7259, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7253-7259.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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