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Journal of Bacteriology, December 2001, p. 7318-7328, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7318-7328.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Global Transcriptional Response of Bacillus subtilis to Heat Shock

John D. Helmann,1,* Ming Fang Winston Wu,2 Phil A. Kobel,2 Francisco-Javier Gamo,3 Michael Wilson,2,dagger Maud M. Morshedi,2 Marc Navre,2 and Chris Paddon2

Department of Microbiology, Cornell University, Ithaca, New York 14853-81011; Affymax Research Institute, Santa Clara, California 950512; and Molecular Microbiology Department, Glaxo Wellcome, SA, C/Severo Ochoa, 28760 Tres Cantos, Spain3

Received 10 July 2001/Accepted 12 September 2001

In response to heat stress, Bacillus subtilis activates the transcription of well over 100 different genes. Many of these genes are members of a general stress response regulon controlled by the secondary sigma factor, sigma B, while others are under control of the HrcA or CtsR heat shock regulators. We have used DNA microarrays to monitor the global transcriptional response to heat shock. We find strong induction of known sigma B-dependent genes with a characteristic rapid induction followed by a return to near prestimulus levels. The HrcA and CtsR regulons are also induced, but with somewhat slower kinetics. Analysis of DNA sequences proximal to newly identified heat-induced genes leads us to propose ~70 additional members of the sigma B regulon. We have also identified numerous heat-induced genes that are not members of known heat shock regulons. Notably, we observe very strong induction of arginine biosynthesis and transport operons. Induction of several genes was confirmed by quantitative reverse transcriptase PCR. In addition, the transcriptional responses measured by microarray hybridization compare favorably with the numerous previous studies of heat shock in this organism. Since many different conditions elicit both specific and general stress responses, knowledge of the heat-induced general stress response reported here will be helpful for interpreting future microarray studies of other stress responses.

* Department of Microbiology, Cornell University, Ithaca, NY 14853-8101. Phone: (607) 255-6570. Fax: (607) 255-3904. E-mail: jdh9{at}cornell.edu.

dagger Present address: Microarray Research Facility, NIAID/NIH, Rockville, MD 20852.

Journal of Bacteriology, December 2001, p. 7318-7328, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7318-7328.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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