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Journal of Bacteriology, December 2001, p. 7329-7340, Vol. 183, No. 24
Genencor International, Palo Alto, California
94304
Received 2 July 2001/Accepted 26 September 2001
The availability of the complete sequence of the Bacillus
subtilis chromosome (F. Kunst et al., Nature 390:249-256,
1997) makes possible the construction of genome-wide DNA arrays and the
study of this organism on a global scale. Because we have a
long-standing interest in the effects of scoC on
late-stage developmental phenomena as they relate to
aprE expression, we studied the genome-wide effects of a
scoC null mutant with the goal of furthering the
understanding of the role of scoC in growth and
developmental processes. In the present work we compared the expression
patterns of isogenic B. subtilis strains, one of which carries a null mutation in the scoC locus
(scoC4). The results obtained indicate that
scoC regulates, either directly or indirectly, the
expression of at least 560 genes in the B. subtilis
genome. ScoC appeared to repress as well as activate gene expression. Changes in expression were observed in genes encoding transport and
binding proteins, those involved in amino acid, carbohydrate, and
nucleotide and/or nucleoside metabolism, and those associated with motility, sporulation, and adaptation to atypical conditions. Changes in gene expression were also observed for transcriptional regulators, along with sigma factors, regulatory phosphatases and
kinases, and members of sensor regulator systems. In this report, we
discuss some of the phenotypes associated with the scoC
mutant in light of the transcriptome changes observed.
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.24.7329-7340.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Correlation between Bacillus subtilis
scoC Phenotype and Gene Expression Determined Using Microarrays
for Transcriptome Analysis
*
Corresponding author. Mailing address: Genencor
International Inc., 925 Page Mill Rd., Palo Alto, CA 94304. Phone:
(650) 846-7538. Fax: (650) 621-7938. E-mail:
eferrari{at}genencor.com.
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