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Journal of Bacteriology, December 2001, p. 7365-7370, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7365-7370.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comprehensive DNA Microarray Analysis of Bacillus subtilis Two-Component Regulatory Systems

Kazuo Kobayashi,1 Mitsuo Ogura,2 Hirotake Yamaguchi,3 Ken-Ichi Yoshida,3 Naotake Ogasawara,1 Teruo Tanaka,2 and Yasutaro Fujita3,*

Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101,1 Department of Marine Science and Technology, Tokai University, Shimizu, Shizuoka 424-8610,2 and Department of Biotechnology, Fukuyama University, Fukuyama, Hiroshima 729-0292,3 Japan

Received 2 July 2001/Accepted 25 August 2001

It has recently been shown through DNA microarray analysis of Bacillus subtilis two-component regulatory systems (DegS-DegU, ComP-ComA, and PhoR-PhoP) that overproduction of a response regulator of the two-component systems in the background of a deficiency of its cognate sensor kinase affects the regulation of genes, including its target ones. The genome-wide effect on gene expression caused by the overproduction was revealed by DNA microarray analysis. In the present work, we newly analyzed 24 two-component systems by means of this strategy, leaving out 8 systems to which it was unlikely to be applicable. This analysis revealed various target gene candidates for these two-component systems. It is especially notable that interesting interactions appeared to take place between several two-component systems. Moreover, the probable functions of some unknown two-component systems were deduced from the list of their target gene candidates. This work is heuristic but provides valuable information for further study toward a comprehensive understanding of the B. subtilis two-component regulatory systems. The DNA microarray data obtained in this work are available at the KEGG Expression Database website (http://www.genome.ad.jp/kegg/expression).

* Corresponding author. Mailing address: Department of Biotechnology, Faculty of Engineering, Fukuyama University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan. Phone: 81 849 36 2111. Fax: 81 849 36 2459. E-mail: yfujita{at}bt.fubt.fukuyama-u.ac.jp.

Journal of Bacteriology, December 2001, p. 7365-7370, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7365-7370.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.