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Journal of Bacteriology, December 2001, p. 7397-7402, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7397-7402.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Mere Lack of rT Modification in Initiator tRNA Does Not Facilitate Formylation-Independent Initiation in Escherichia coli

Swapna Thanedar, T. K. Dineshkumar, and Umesh Varshney*

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India

Received 2 July 2001/Accepted 18 September 2001

Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA may facilitate initiation in the absence of formylation. In this report we show, by using trmA strains of Escherichia coli (defective for rT modification) and a sensitive in vivo initiation assay system, that the lack of rT modification in the initiators is not sufficient to effect formylation-independent initiation of protein synthesis.

* Corresponding author. Mailing address: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India. Phone: 91-80-309-2686. Fax: 91-80-360-2697. E-mail: varshney{at}mcbl.iisc.ernet.in.

Journal of Bacteriology, December 2001, p. 7397-7402, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7397-7402.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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  • Dineshkumar, T. K., Thanedar, S., Subbulakshmi, C., Varshney, U. (2002). An unexpected absence of queuosine modification in the tRNAs of an Escherichia coli B strain. Microbiology 148: 3779-3787 [Abstract] [Full Text]  


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