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Journal of Bacteriology, February 2001, p. 1085-1089, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1085-1089.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

umuDC-dnaQ Interaction and Its Implications for Cell Cycle Regulation and SOS Mutagenesis in Escherichia coli

Mark D. Sutton, Sumati Murli, Timothy Opperman, Carly Klein,^§ and Graham C. Walker^*

Biology Department, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Received 15 September 2000/Accepted 20 October 2000

The Escherichia coli SOS-regulated umuDC gene products participate in a DNA damage checkpoint control and in translesion DNA synthesis. Specific interactions involving the UmuD and UmuD' proteins, both encoded by the umuD gene, and components of the replicative DNA polymerase, Pol III, appear to be important for regulating these two biological activities of the umuDC gene products. Here we show that overproduction of the  proofreading subunit of Pol III suppresses the cold sensitivity normally associated with overexpression of the umuDC gene products. Our results suggest that this suppression is attributable to specific interactions between UmuD or UmuD' and the C-terminal domain of .

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^* Corresponding author. Mailing address: Biology Department, 68-633, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139. Phone: (617) 253-6716. Fax: (617) 253-2643. E-mail: gwalker{at}MIT.EDU.

Present address: Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536-0812.

Present address: Department of Pathogen Genetics, Genome Therapeutics Corporation Waltham, MA 02453.

^§ Present address: Department of Biochemistry, University of California, San Francisco, San Francisco, CA 94143-0448.

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Journal of Bacteriology, February 2001, p. 1085-1089, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1085-1089.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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